Axio observer z1 7 wide

Yeast cultures were grown as described above and concentrated by brief centrifugation. A total of 1 μL of cell suspension was immobilized on a 0.17-mm cover glass by a thin film of 1% agarose prepared in 50 mM potassium phosphate buffer (pH 6.3). For time-lapse experiments of exponential cultures, the 1% agarose was prepared in fresh SC medium to supplement nutrients. S. cerevisiae cells were imaged using Zeiss LSM 880 and Olympus BX61WI scanning confocal microscopes. For C. albicans cells, 9- to 11-layered z-stacks were made using a Zeiss Axio Observer Z1/7 wide-field fluorescence microscope. All microscopes were equipped with a 100× PlanApochromat oil immersion objective (numerical aperture [NA], 1.4). GFP and FM4-64 were excited using the 488- and 514-nm lines of the Argon laser, respectively. mCherry/dsRed/mRFP were excited using the 561-nm DPSS laser. C. albicans images were drift corrected and deconvolved in the proprietary Zeiss ZEN software, Black edition.

Yeast cultures were grown as described above and concentrated by brief centrifugation. A total of 1 μL of cell suspension was immobilized on a 0.17-mm cover glass by a thin film of 1% agarose prepared in 50 mM potassium phosphate buffer (pH 6.3). For time-lapse experiments of exponential cultures, the 1% agarose was prepared in fresh SC medium to supplement nutrients. S. cerevisiae cells were imaged using Zeiss LSM 880 and Olympus BX61WI scanning confocal microscopes. For C. albicans cells, 9- to 11-layered z-stacks were made using a Zeiss Axio Observer Z1/7 wide-field fluorescence microscope. All microscopes were equipped with a 100× PlanApochromat oil immersion objective (numerical aperture [NA], 1.4). GFP and FM4-64 were excited using the 488- and 514-nm lines of the Argon laser, respectively. mCherry/dsRed/mRFP were excited using the 561-nm DPSS laser. C. albicans images were drift corrected and deconvolved in the proprietary Zeiss ZEN software, Black edition.