Assay kits of blood urea nitrogen

Dulbecco’s Modified Eagle’s Medium (DMEM) was purchased from KeyGEN (Jiangsu, China). The assay kits of blood urea nitrogen (BUN), creatinine (Cr), total cholesterol (TC), triglyceride (TG), and free fatty acids (FFAs) were obtained from Nanjing Jiancheng Institute of Biotechnology (Nanjing, China). Tissue Protein Extraction Kit and the Bicinchoninic acid (BCA) Protein Assay Kit were purchased from Beyotime Institute of Biotechnology (Jiangsu, China). Tris, sodium dodecyl sulfate (SDS) and 4,6-diamidino-2-phenylindole (DAPI) were purchased from Sigma (St. Louis, MO, USA). Lipopolysaccharide (LPS) and Oil red O staining solution were purchased from Solarbio Technology Co., Ltd (Beijing, China) and KeyGEN (Jiangsu, China). CCK-8 was purchased from Selleck Chemicals (Houston, USA). Lipofectamine 2000 was obtained from GenePharma (Shanghai, China). RNAisoPlus, PrimeScript®RT reagent Kit with DNA Eraser (Perfect Real Time) and SYBR®Premix Ex TaqTMII (Tli RNaseH Plus) were purchased from TaKaRa Biotechnology Co., Ltd. (Dalian, China). FABP4 siRNA was purchased from RIBOBIO Co., Ltd. (Guangzhou, China). Mouse and Rat FABP4 ELISA Kits were purchased from Boster Biological Technology Co., Ltd. (California, USA). FABP4 inhibitor BMS309403 was purchased from MedChemExpress (Shanghai, China).

DEAE‐Sepharose^TM Fast Flow column was purchased from General Electric Company, Inc (Connecticut, USA). DDP was purchased from Qilu Pharmaceutical Co. Ltd (Shandong, China). The assay kits of blood urea nitrogen (BUN), serum creatinine (CRE), superoxide dismutase (SOD), malondialdehyde (MDA), and glutathione peroxidase (GSH‐PX) were purchased from Nanjing Jiancheng Bioengineering Institute Co. Ltd (Nanjing, China). The micro blood calcium/phosphorus (Ca/P) assay kit was purchased from Solarbio Life Science Co. Ltd (Beijing, China). The primary rabbit and mouse antibodies including NADPH oxidase 4 (NOX4), interleukin‐1 beta (IL‐1β), tumor necrosis factor‐alpha (TNF‐α), caspase‐3, cleaved caspase‐3, caspase‐8, caspase‐9, Bax, Bcl‐2, β‐actin, mitogen‐activated protein kinase 8 (MAPK8, JNK), p‐JNK, mitogen‐activated protein kinase 14 (MAPK14, p38), p‐p38, extracellular regulated protein kinases (ERK1/2), p‐ERK1/2, secondary goat anti‐rabbit, and goat anti‐mouse were purchased from Proteintech Group, Inc (Chicago, USA) and Thermo Fisher Scientific, Inc (Massachusetts, USA). TUNEL kit was purchased from Sigma‐Aldrich, Inc (San Francisco, USA).