Sybr premix ex tap reagent kit

Post-transfection with decorin A and decorin B, RNAiso Plus (Takara) was used to isolate total RNA from the PC cells following the manufacturer’s guidelines. Next, the Prime Script RT reagent kit (Takara) was used to reverse transcribe the RNA to cDNA. Semi-quantitative RT PCR was used to confirm the expression of decorin A and B after transfected while qRT-PCR was used to determine the level of expression of cell proliferation-related genes (PCNA, cyclin A, cyclin B, and cyclin D) and apoptosis-related genes (BCL2, BAX, and p53) using the SYBR Premix Ex Tap reagent kit (Takara, China) with β-actin as the internal reference. The -ΔΔCq method was used to calculate the expression level of target genes.

Total RNA was extracted with TRIzol reagent (Invitrogen). cDNA was generated from RNA template using a reverse transcription kit (Invitrogen). qRT-PCR analysis was performed by SYBR® Premix Ex Tap Reagent kit (TaKaRa, Dalian, China) [17 (link)]. β-actin was used for the normalization of RBM8A. The primer sequences are shown in Table 1. The relative expressions were calculated by using 2^−ΔΔCt method.