Chlorin e6 ce6

As antimicrobial agents Chlorin e6 (Ce6) and Pheophorbide a (Pheo) (Santa Cruz Biotechnology Inc., Dallas, TX, USA) were selected. Their solutions were prepared in PBS (phosphate-buffered saline, ThermoFisher Scientific, Waltham, MA, USA) pH = 7.4 to obtain the maximum of the Soret band in the range 405–407 nm for luminescence measurements/microscopic observations and the strong Q-band with the maximum in the range 650–670 nm for photodynamic bacteria inactivation under laser light exposure. The photoactive materials were prepared from 20 µL of the 0.1% aq. ammonium acryloyldimethyltaurate copolymer (AVC) (Clariant International LTD., Muttenz, Switzerland) and deposited onto glass-bottom micro-dishes (µ-Dish 35 mm low, Ibidi GmbH, Gräfelfing, Germany). Photosensitizer (PS) doped materials were prepared from AVC and photosensitizer’s solutions to obtain the 17 nmol of the PSs in each sample. The area occupied by each material was equal to 30 mm².

Chlorin e6 (Ce6, MW = 596.68) was purchased from Santa Cruz Biotechnology, iron (III) acetylacetonate, 1,2-hexadecanediol, benzyl ether, oleylamine, and anhydrous dimethyl sulfoxide (DMSO) were obtained from Aldrich. Oleic acid was purchased from Chem-Impex International, Inc. Ethanol was purchased from Decon Labs, Inc. All other chemicals and solvents were of analytical grade and were used without further purification. All the buffer solutions were prepared with deionized water.

All the reagents including 1-octadecene (ODE, 90%), oleic acid (OA, 90%), ethanol (EtOH) and methanol (MeOH), dimethylformamide (DMF), chloroform, milli-Q water, tetrahydrofuran (THF), yttrium(III) chloride anhydrous (YCl₃, 99.9%), ytterbium(III) chloride anhydrous (YbCl₃, 99.9%), erbium(III) chloride anhydrous (ErCl₃, 99.9%), ammonium fluoride (NH₄F, 96%), Sodium hydroxide (NaOH, 96%), Sodium trifluoroacetate (Na-TFA, 98%), neodymium(III) chloride (NdCl₃ 99.9%), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), N-hydroxysuccinimide (s-NHS), Rose Bengal (RB), 6-bromohexanoic acid (Br-HA), poly(isobutylene-alt-maleic anhydride), dodecylamine (DDA), dibenzocyclooctine-amine (DBCO-NH₂), 1,3-Diphenylisobenzofuran (DPBF, 97%) were purchased from Sigma-Aldrich. Chlorin e6 (Ce6) was purchased from Santa Cruz Biotechnology. 3-azido-propylamine (NH₂-N₃) was purchased from Fluorochem Ltd.