The treated TG905 cells were washed thrice with ice-cold PBS and homogenized with RIPA lysate containing 1 mM phenylmethanesulfonyl fluoride. Samples were centrifuged at 12000 rpm for 10 min, and the supernatant were collected. The total protein concentration was measured using BCA protein assay kits (Solabio). Cell lysates were separated using 10% SDS-PAGE and electro-transferred onto a polyvinylidene difluoride membrane. After incubation in blocking buffer (1×TBS, 0.1% Tween-20 and 5% w/v dry nonfat milk) at 4℃, membranes were incubated with the desired primary antibody, followed by incubation with the appropriate peroxidase-conjugated secondary antibody. The primary antibodies included those for Nrf2 (16396-1-AP, Proteintech, Wuhan, China), HO-1 (ab189491, Abcam, Cambridge, England), DMT1 (20507-1-AP, Proteintech), GPX4 (ab125006, Abcam), ferritin (ab75973, Abcam), transferrin receptor (ab214039, Abcam), ferroportin-1 (bs4906R, Bioss, Beijing, China), and GAPDH(ab181602, Abcam). The films were scanned, and the intensity values of each band were determined using an Alphalmager HP system (Cell Biosciences Inc., Santa Clara, CA, USA).
Bs4906r
Manufactured by Bioss Antibodies
Sourced in China
Bs4906R is a high-precision laboratory equipment designed for specific applications. It functions as a core component in various scientific experiments and analyses, providing accurate and reliable measurements. The detailed specifications and intended uses of this product are not available for an unbiased, factual description.
Automatically generated - may contain errors
Lab products found in correlation
Ab75973, by Abcam (1 mentions)
Ab189491, by Abcam (1 mentions)
Ab125006, by Abcam (1 mentions)
Ab181602, by Abcam (1 mentions)
Ab214039, by Abcam (1 mentions)
Ab183781, by Abcam (1 mentions)
Ab269514, by Abcam (1 mentions)
Ab155282, by Abcam (1 mentions)
Ab125066, by Abcam (1 mentions)
Df12509, by Affinity Biosciences (1 mentions)
β actin, by ZenBio (1 mentions)
2 protocols using bs4906r
1
Nrf2-HO-1 Pathway in Iron Homeostasis
2
Western Blot Analysis of Ferroptosis Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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After the total protein was extracted, the protein concentration was examined. With 50 μg of total protein, each protein sample was separated for electrophoresis on 8–12% sodium dodecyl sulfate–polyacrylamide gels and transferred onto polyvinylidene difluoride membranes. Then, the membranes would be blocked for 2 h at room temperature and incubated with the primary antibody against FPN1 (bs-4906R, Bioss, China), GPX4 (ab125066, Abcam, UK), ACSL4 (ab155282, Abcam, UK), FTH1 (ab183781, Abcam, UK), TFR1 (ab269514, Abcam, UK), xCT (DF12509, Affinity Bioscience, China), 4-HNE (GTX01087, GeneTex, USA), Nox4 (380,874, Zen-bioscience, China), and β-actin (380,624, Zen-bioscience, China) at 4 °C overnight. Then, the membranes were incubated with the second antibodies (Zen-bioscience, China) for 1 h at room temperature. The target bands were visualized by enhanced chemiluminescence and analyzed on Chemiluminescent gel imaging (MiniChemi 610 Plus, Beijing Saizhi Venture Technology Co., Ltd.), which were then quantified by ImageJ analysis software (National Institutes of Health, Bethesda, MD).
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