Hand gal4

Manufactured by Bloomington Drosophila Stock Center

The Hand-Gal4 is a genetic tool used in Drosophila research. It is a tool for targeted gene expression, allowing researchers to drive the expression of genes of interest in the Drosophila hand-bearing appendages.

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Lab products found in correlation

Uas mcherry, by Bloomington Drosophila Stock Center (2 mentions) Uas mcherry nls, by Bloomington Drosophila Stock Center (2 mentions) Uas cd8 gfp, by Bloomington Drosophila Stock Center (1 mentions) Uas gfp nls, by Bloomington Drosophila Stock Center (1 mentions) Uas redstinger, by BD (1 mentions) 10xuas myr gfp, by BD (1 mentions)

3 protocols using hand gal4

Drosophila Genetic Toolkit Protocols

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Flies were kept at 25 °C 12-hour light/dark cycles on a standard cornmeal medium. 10xUAS-myr::GFP (BDSC #32197), UAS-RedStinger (#8546), Tret1–1-Gal4 (#67471), Vmat-Gal4 (#66806), rost::LacZ (#12042), CG3036::GFP (#50804), b-Gal4 (#76724), DAT-Gal4 (#84622), CG31663-Gal4 (#76708), ome-Gal4 (#76166), Canton-S (#64349), Hand-Gal4 (#66795), CG4288-Gal4::GFP (#29208), alphaTub85E::GFP (#60267), Npc2b-Gal4::GFP (#24694), Adgf-D-Gal4 (#77799), R25A01-Gal4 (#49102), UASCD8:GFP (from Bloomington), Cyp4g15-Gal4 (#39103), GstT4-Gal4 (#77777), nkd::LacZ (#25111), R94A08-Gal4 (#40673), UAS-GFP.nls (#4776), UAS-sfGFPnlsPEST-2A-B2ase – this is an unpublished line developed by Dr. Benjamin Choi (Desplan lab), consisting of a destabilized nuclear-localized GFP (Table Fly Genotypes).

Ferreira A.A, & Desplan C. (2023). An Atlas of the Developing Drosophila Visual System Glia and Subcellular mRNA Localization of Transcripts in Single Cells. bioRxiv.

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Drosophila Live Imaging Markers

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We used the following markers for live imaging: E-cadherin:mKate2x3 (Pinheiro et al., 2017) , hand-hand:GFP (Han and Olson, 2005) , mid-mid E19 :GFP (Jin et al., 2013) , hand-GFP:moesinABD (Haack et al., 2014) , UAS-dlg1:GFP (Koh et al., 1999) , UAS-mCherry:moesinABD (Millard and Martin, 2008) , UAS-mCherry:nls (Bloomington Drosophila Stock Center #38424), and UAS-sqh:GFP (gift of E. Caussinus). UAS constructs were driven with hand-Gal4 (Bloomington Drosophila Stock Center #48396), or tinC∆4-Gal4 (Lo and Frasch, 2001) .

Balaghi N., Erdemci-Tandogan G., McFaul C, & Fernandez-Gonzalez R. (2023). Myosin waves and a mechanical asymmetry guide the oscillatory migration of Drosophila cardiac progenitors. Developmental cell, 58(14).

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Drosophila Live Imaging Markers

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Balaghi N., Erdemci-Tandogan G., McFaul C., & Fernández‐González R. (2022). Myosin waves and a mechanical asymmetry guide the oscillatory migration of Drosophila cardiac progenitors.

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