The volatile flavor compounds extracted by HS-SPME were analyzed by GC/MS (Agilent 6890 gas chromatography coupled with a 5973 mass selective detector, Agilent Co., Palo Alto, CA, USA) using a Stabilwax®-DA column (30 m length × 0.25 mm i.d. × 0.25 μm film thickness: Restek, Bellefonte, PA, USA). The oven temperature was kept constant at 40 °C for 3 min, after which it was increased to 210 °C at intervals of 4 °C/min. The injector temperature was held at 250 °C for the analysis of the split-less mode, and the flow rate of the carrier gas was held at 0.8 mL/min using helium. MSD (Agilent 5975C) conditions (capillary direct interface temperature 250 °C, ion source temperature 230 °C, EI ionization voltage 70 eV, mass range 40–550 atomic mass unit (amu) and scan rate 2.2 scans/s) were used, and the identification of the volatile compounds was confirmed by comparing the retention indices (RI), aromatic spectra (NIST05A), and aromatic properties.
Agilent 6890 gas
Manufactured by Agilent Technologies
Sourced in United States
The Agilent 6890 gas chromatograph is a versatile analytical instrument used for the separation and identification of complex chemical mixtures. It features a highly sensitive and accurate detection system, enabling precise quantification of sample components. The 6890 gas chromatograph is designed to provide reliable and reproducible results in a wide range of applications, including environmental analysis, food and beverage testing, and pharmaceutical research.
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2 protocols using agilent 6890 gas
1
GC-MS Analysis of Volatile Compounds
2
Sterols Identification by GC-MS
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Chromatographic separation was performed on an Agilent 6890 gas chromatograph (GC) coupled to a 5973 quadrupole mass spectrometer (MS) from Agilent Technologies, USA. The injector was programmed at 250 ºC and a splitless injection mode was used. Aliquots of 2 µL of the sample dissolved in hexane were injected into GC-MS. Separation of the sterols was carried out using HP-5-MS 30 m x 0.25 mm i.d. x 0.5 µm column (Agilent Technologies, USA). The carrier gas was helium, at a constant flow rate of 1 mL min -1 . The GC oven temperature started at 150 ºC, and then was ramped to 250ºC at 40 ºC min -1 . Next, the temperature was raised to 300 ºC at 5 ºC min - 1 and held there for 3.5 min. The source temperature was fixed at 280 ºC and MS worked in electron impact mode (EI, 70 eV). In order to avoid overloading the GC-MS with hexane, a solvent delay of 4 min was implemented. The mass spectra were acquired over the mass-to-charge (m/z) range of 50-600. The SIM conditions for the neutral lipids were determined after the identification of the most abundant and characteristic ions in SCAN conditions. Integration was performed with Chemstation integrator.
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