The following antibodies were purchased: rabbit polyclonal anti‐Sirt2 antibody (Cat#: AV32384; Sigma); mouse monoclonal FITC‐conjugated anti‐α‐tubulin antibody (Cat#: F2168; Sigma); mouse monoclonal anti‐Myc antibody (Cat#: ab18185; Abcam); human anticentromere CREST antibody (Cat#: 15‐234; Antibodies Incorporated); Cy5‐conjugated donkey anti‐human IgG (Cat#: 709‐605‐149 and 705‐095‐147; Jackson ImmunoResearch Laboratory).
Mouse monoclonal fitc conjugated anti α tubulin antibody
Manufactured by Merck Group
Sourced in United States
The Mouse monoclonal FITC‐conjugated anti‐α‐tubulin antibody is a laboratory reagent used to detect and visualize the alpha-tubulin component of the cytoskeleton in cell samples. The antibody is conjugated with the fluorescent dye FITC, allowing for fluorescent detection of alpha-tubulin in microscopy or flow cytometry applications.
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Lab products found in correlation
Cy5 conjugated donkey anti human igg, by Jackson ImmunoResearch (5 mentions)
Fitc conjugated goat anti rabbit igg, by Thermo Fisher Scientific (4 mentions)
Fitc conjugated donkey anti goat igg, by Jackson ImmunoResearch (4 mentions)
Mouse monoclonal anti β actin antibody, by Merck Group (3 mentions)
Mouse monoclonal anti myc antibody, by Abcam (2 mentions)
Sheep polyclonal anti bubr1 antibody, by Abcam (2 mentions)
Mouse monoclonal anti myc tag antibody, by Abcam (2 mentions)
Goat polyclonal anti bubr1 antibodies, by Abcam (2 mentions)
Rabbit polyclonal anti sirt2 antibody, by Merck Group (1 mentions)
Rabbit monoclonal anti myc antibody, by Cell Signaling Technology (1 mentions)
Milrinone, by Cayman Chemical (1 mentions)
Nocodazole, by Merck Group (1 mentions)
Tritc conjugated phalloidin, by Merck Group (1 mentions)
Anti β actin monoclonal antibody, by Abcam (1 mentions)
Taxol, by Merck Group (1 mentions)
M2 medium, by Merck Group (1 mentions)
Normal rabbit igg, by Santa Cruz Biotechnology (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (1 mentions)
Rabbit anti phospho histone h2a x, by Cell Signaling Technology (1 mentions)
Fluorescence microscope, by Zeiss (1 mentions)
10 protocols using mouse monoclonal fitc conjugated anti α tubulin antibody
1
Antibody Immunofluorescence Staining Protocol
2
Antibody Characterization in Cell Studies
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The following antibodies were used in this study: rabbit polyclonal anti‐SIRT3 (Cat#:ab8667; Abcam); rabbit monoclonal anti‐GSK3β (Cat#:ab32391; Abcam); mouse monoclonal FITC‐conjugated anti‐α‐tubulin antibody (Cat#: F2168; Sigma); rabbit monoclonal anti‐Myc antibody (Cat#: 2278; Cell Signaling Technology); mouse monoclonal HRP‐conjugated anti‐α‐tubulin antibody (Cat#: HRP‐66031; Proteintech).
3
Antibody Procurement for Cytoskeletal Research
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A rabbit polyclonal anti-KIF2A antibody was purchased from Novus Biologicals (Littleton, CO, USA). Normal rabbit IgG was purchased from Santa Cruz Biotechnology (Dallas, CA, USA). A rabbit polyclonal anti-SKA1 antibody was purchased from Abcam (Cambridge, MA, USA). A rabbit monoclonal anti-TACC3 antibody was purchased from Abcam (Cambridge, MA, USA). Mouse monoclonal FITC-conjugated anti-α-tubulin antibody, TRITC-conjugated phalloidin, nocodazole and taxol were purchased from Sigma-Aldrich (St Louis, MO, USA). Sheep polyclonal anti-BubR1 antibody was purchased from Abcam (Cambridge, MA, USA). Alexa Fluor 546-conjugated donkey anti-sheep and donkey anti-rabbit antibodies were obtained from Invitrogen (Carlsbad, CA, USA). M2 medium was purchased from Sigma-Aldrich (St Louis, MO, USA). Milrinone was obtained from Cayman Chemical Co. (Ann Arbor, MI, USA). Mounting medium containing DAPI was obtained from Vector Laboratories (Burlingame, CA, USA). Horseradish peroxidase (HRP)-conjugated mouse anti-rabbit IgG and horseradish peroxidase (HRP)-conjugated rabbit anti-mouse IgG were obtained from Zhong Shan Jin Qiao Co. (Beijing, P. R. China). A mouse monoclonal anti-β-actin antibody was obtained from Abcam (Cambridge, MA, USA). Enhanced chemiluminescence (ECL) detection substrate was obtained from Xiamen Lulong Biotech Co. (Xiamen, P. R. China).
4
Immunofluorescence Staining of Cellular Proteins
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Rabbit polyclonal anti-FKBP25 antibody were purchased from Abcam (Cat#: ab16654; 1:150); Mouse monoclonal FITC conjugated anti α-tubulin antibody were purchased from Sigma (Cat#: F2168; 1:500); human anti-centromere CREST antibody was purchased from Antibodies Incorporated (Cat#: 15234; 1:500); Cy5-conjugated donkey anti-human IgG and FITC-conjugated donkey anti-goat IgG were purchased from Jackson Immuno-Research Laboratory (Cat#: 709605149 and 705095147; 1:500); Goat polyclonal anti-BubR1 antibody and mouse monoclonal anti-Myc tag antibody were purchased from Abcam (Cat#: ab28193 and ab18185; 1:250); and FITC conjugated goat anti-rabbit IgG purchased from Thermo Fisher Scientific (1:300).
5
Immunofluorescence Staining of Oocytes
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Oocytes were fixed in 3.7% paraformaldehyde dissolved in polyvinyl alcohol in PBS (PVA-PBS) for 30 min at room temperature or overnight at 4°C. For permeabilization, fixed oocytes were transferred into 0.5% Triton X-100 for 1 h at room temperature. After blocking in 1% BSA in PBS for 1 h, oocytes were stained with the primary antibodies rabbit anti-phospho-Histone H2A.X (1:200; #9718T; Cell Signaling Technology, Danvers, MA) and mouse anti-pericentrin (1:200; #611814; BD Biosciences, Franklin Lakes, NJ) at 4°C overnight. After washing the oocytes three times with PVA-PBS, they were labeled with secondary antibodies Alexa Fluor 594 goat anti-rabbit and Alexa Fluor 488 goat anti-mouse (1:200; #A11012; #A11001, respectively; Thermo Fisher Scientific) at room temperature for 2 h. For spindle staining, the oocytes were incubated with mouse monoclonal FITC-conjugated anti-α-tubulin antibody (1:200; Sigma-Aldrich) for 2 h at room temperature. Stained oocytes were washed extensively with PVA-PBS and incubated with Hoechst 33342 (10 μg/mL; #B2261; Sigma-Aldrich) for 20 min. After washing, the samples were mounted on glass slides in Vectashield containing DAPI (Vector Laboratories, Burlingame, CA) and observed under a fluorescence microscope (Zeiss, Oberkochen, Germany).
6
Antibody Validation for SIRT4, Tubulin, and Actin
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Rabbit polyclonal anti‐SIRT4 antibodies (Cat#: S0948), mouse monoclonal FITC‐conjugated anti‐α‐tubulin antibodies (Cat#: F2168), and mouse monoclonal anti‐β‐actin antibodies (Cat#: A5441) were purchased from Sigma; mouse monoclonal anti‐Myc antibodies (Cat#: ab18185) were purchased from Abcam; Rabbit polyclonal anti‐PDHE1α (pSerine232) antibodies and Rabbit polyclonal anti‐PDHE1α (pSerine293) antibodies were purchased from EMD Chemicals (Cat#: AP1063 and AP1062).
7
Antibody Reagents for Protein Detection
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Mouse polyclonal anti‐SIRT1 antibodies (Cat#: S5196), anti‐H4K16ac antibodies (Cat#: ab109463), and mouse monoclonal anti‐NMNAT2 antibodies (Cat#: ab56980) were purchased from Abcam (Cambridge, MA, UK); Myc antibodies were purchased from Cell signaling Technology (Cat#: 2278); mouse monoclonal FITC‐conjugated anti‐α‐tubulin antibodies (Cat#: F2168) and mouse monoclonal anti‐β‐actin antibodies (Cat#: A5441) were purchased from Sigma; Cy5‐conjugated goat anti‐human IgG was purchased from Jackson Immuno Research Laboratory (West Grove, PA, USA).
8
Immunofluorescence Staining of Cellular Proteins
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Rabbit polyclonal anti‐HDAC3 antibodies were purchased from Santa Cruz Biotechnology (Cat#: sc‐376957; 1:150); Mouse polyclonal anti‐acetyl‐tubulin (Lys‐40) antibodies were purchased from Sigma (Cat#: T7451; 1:200); Mouse monoclonal FITC‐conjugated anti‐α‐tubulin antibodies were purchased from Sigma (Cat#: F2168; 1:500). Mouse monoclonal anti‐HDAC3 antibodies were purchased from Cell Signaling Technology (Cat#: 3949T; 1:1,000); human anti‐centromere CREST antibody was purchased from Antibodies Incorporated (Davis, CA, USA; Cat#: 15–234; 1:500); Cy5‐conjugated donkey anti‐human IgG and FITC‐conjugated donkey anti‐goat IgG were purchased from Jackson ImmunoResearch Laboratory (Cat#: 709–605–149 and 705–095–147; 1:500); goat polyclonal anti‐BubR1 antibodies and mouse monoclonal anti‐Myc tag antibody were purchased from Abcam (Cat#: ab28193 and ab18185; 1:250); FITC‐conjugated goat anti‐rabbit IgG purchased from Thermo Fisher Scientific (1:300).
9
Immunofluorescence Staining Protocol
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Rabbit polyclonal anti-ASB7 antibodies were purchased from Novus Biologicals USA (Cat#: NBP1-86157; 1:250). Mouse monoclonal FITC-conjugated anti-α-tubulin antibodies were purchased from Sigma (Cat#: F2168; 1:500). Human anti-centromere CREST antibody was purchased from Antibodies Incorporated (Cat#: 15234; 1:500). Cy5 conjugated donkey anti-human IgG and FITC-conjugated donkey anti-goat IgG were purchased from Jackson ImmunoResearch Laboratory (Cat#: 709605149 and 705095147; 1:500). Goat polyclonal anti-BubR1 antibodies and mouse monoclonal anti-Myc-tag antibody were purchased from Abcam (Cat#: ab28193 and ab18185; 1:250). FITC-conjugated goat anti-rabbit IgG was purchased from Thermo Fisher Scientific (1:300).
10
Immunofluorescence Labeling of Cellular Proteins
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Rabbit polyclonal anti-Rab6a and Sheep polyclonal anti-BubR1 antibodies were purchased from Abcam (Cambridge, MA, USA; Cat#: ab95954 and ab28193); Human anti-centromere CREST antibody was purchased from Antibodies Incorporated (Davis, CA, USA; Cat#:15–234). Cy5-conjugated donkey anti-human IgG and FITC-conjugated donkey anti-goat IgG were purchased from Jackson ImmunoResearch Laboratory (West Grove, PA, USA; Cat#: 709-605-149 and 705-095-147). FITC-conjugated goat anti-rabbit IgG was purchased from Thermo Fisher Scientific (Rockford, IL, USA). Mouse monoclonal anti-β-actin antibodies and mouse monoclonal FITC-conjugated anti-α-tubulin antibodies were purchased from Sigma (St. Louis, MO, USA; Cat#: A5441 and F2168).
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