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3 protocols using antibody against tata binding protein

1

Molecular Signaling Pathway Analysis

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Dulbecco's Modified Eagle Medium (DMEM), fetal bovine serum (FBS), penicillin/streptomycin and Alexa Fluor 488-conjugated goat anti-rabbit antibodies were obtained from Invitrogen (Carlsbad, CA, USA). LY294002, Wortmannin and diphenyleneiodium (DPI) were purchased from Calbiochem (La Jolla, CA, USA). Antibodies against extracellular signal-regulated kinase (ERK), phospho-ERK, Akt, phospho-Akt, nuclear factor κB p65 (NF-κB p65) and inhibitor of κBα (IκBα) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against p38, phospho-p38 and inducible nitric oxide synthase (iNOS) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibody against TATA binding protein (TBP) was purchased from Abcam (Cambridge, UK). Antibody against gp91phox was purchased from BD biosciences (San Jose, CA, USA). Horseradish peroxide (HRP)-conjugated immunoglobulin G (IgG) antibody was purchased from Vector Laboratories (Burlingame, MA, USA). All the other reagents were purchased from Sigma (St. Louis, MO, USA), unless indicated.
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2

Molecular Mechanisms in Cell Signaling

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PG, avidin-tetramethylrhodamine isothiocyanate (avidin-TRITC), and anti-actin antibody were purchased from Sigma Chemical Company (St. Louis, MO, USA). The antibodies against Ogg1, Nrf2, Erk, and phospho-Erk were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The antibodies against phospho-Akt, Akt, and histone 3 were purchased from Cell Signaling Technology (Danvers, MA, USA). The antibody against TATA-binding protein (TBP) was purchased from Abcam (Cambridge, UK). Thiazolyl blue tetrazolium bromide (MTT) and dimethyl sulfoxide (DMSO) were purchased from Amresco LLC (Solon, OH, USA).
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3

Oxidative Stress and Autophagy Modulation

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OXT, N-acetylcysteine (NAC), Hoechst 33342, 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA), and actin antibodies were obtained from Sigma-Aldrich (St. Louis, MO, USA). Thiazolyl blue tetrazolium bromide (MTT) was purchased from Amresco (Solon, OH, USA). Acridine orange was purchased from Invitrogen (Madison, WI, USA). Antibody against Atg5 (C-term) was purchased from Abgent (San Diego, CA, USA). Antibodies against beclin-1, Atg7, LC3, p62, and phosphorylated Nrf2 (phospho-Nrf2) were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against Nrf2 and heme oxygenase-1 (HO-1) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibody against TATA-binding protein (TBP) was purchased from Abcam (Cambridge, UK). Antibody against Cu/Zn superoxide dismutase (SOD) was purchased from Biodesign International (Saco, ME, USA). All other chemicals and reagents used were of analytical grade.
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