Creatinine auto analyzer

Serum creatinine levels were analyzed using an enzymatic method, a Hitachi creatinine auto-analyzer (model 7170; Hitachi, Tokyo, Japan), and an enzyme solution (Preauto-SCrE-N; Daiichi Pure Chemicals Co., Tokyo, Japan). The serum levels of T chol, LDL-C, and HDL-C were measured by direct enzymatic assays using an automatic analyzer (Hitachi, Tokyo, Japan). The serum levels of the total, HMW-, MMW-, LMW-ADPN, and CTRP-9 fractions were measured using a sensitive enzyme-linked immunosorbent assay kit (SEKISUI MEDICAL Co., Tokyo, Japan). Renal function was evaluated based on the eGFR (= 194 x SCr^-1.094 x age^-0.287 x 0.739 for females, ml/min/1.73 m²), which was calculated based on the serum creatinine (SCr) level, as described previously [12 (link)].
We evaluated calcification of the abdominal aorta using the ACAI. The ACAI was calculated based on assessments of computed tomography scans of the abdominal aortic wall (slice thickness: 5 mm or 10 mm) in the region of interest. Specifically, it was calculated by assessing the percentage of the aortic wall occupied by calcification on each slice and then dividing the sum of the percentage values for all slices by the number of slices (S1 Fig). These analyses were conducted using image analysis software (MITANI Co., Ltd., Fukui, Japan).

Serum creatinine levels were analyzed using an enzymatic method, a Hitachi creatinine auto-analyzer (model 7170; Hitachi, Tokyo, Japan), and an enzyme solution (Preauto-SCrE-N; Daiichi Pure Chemicals Co., Tokyo, Japan). The serum levels of T chol, LDL-C, and HDL-C were measured by direct enzymatic assays using an automatic analyzer (Hitachi, Tokyo, Japan). The serum levels of the total, HMW-, MMW-, and LMW-ADPN fractions were measured using a sensitive enzyme-linked immunosorbent assay kit (SEKISUI MEDICAL Co., Tokyo, Japan). Renal function was evaluated based on the eGFR.
The eGFR (= 194 x SCr^-1.094 x age^-0.287 x 0.739 for females, ml/min/1.73m²) was calculated based on the patients’ serum creatinine (SCr) levels, as described previously [23 (link)].
We evaluated the calcification of the abdominal aorta using the ACAI. The ACAI was calculated based on assessments of computed tomography scans of the abdominal aortic wall (slice thickness: 5 mm or 10 mm) in the region of interest. Specifically, it was calculated by assessing the percentage of the aortic wall occupied by calcification on each slice and then dividing the sum of the percentage values for all slices by the number of slices (S1 Fig). These analyses were conducted using image analysis software (MITANI Co., Ltd., Fukui, Japan).

Serum creatinine levels were measured by means of an isotope dilution mass spectrometry (IDMS) traceable assays both at PGI (modified Jaffe’s method using Cobas C111 auto-analyzer, Roche Diagnostics Limited) and in a central lab in Japan (enzymatic creatinine assay [5 (link)] using Hitachi creatinine auto-analyzer, model 7170, Hitachi). Serum Cys levels were measured in a central lab by colloidal gold immunoassay (Alfresa Pharma, http://www.alfresa-pharma.co.jp) which has been standardized for measurement of cystatin C traceable to ERM-DA471/IFCC [10 (link)].