HEKn were cultured until the third passage before treatment. Keratinocytes were plated in a 96-well plate and at 80% confluence, 10−7 M secosteroids (or 0.1% ethanol control) were added and cells were incubated for 48 h. Cells were fixed and stained with anti-involucrin (1:20, GTX-14504; Genetex, Irvine, CA, USA) anti-CK10 (1:300, Santa Cruz Biotechnology), anti-catalase (1:200, Santa Cruz Biotechnology) or anti-CK14 antibody with Alexa Fluor® 488 (1:500, Santa Cruz Biotechnology) and imaged at 10× magnification in Cytation 5 reader as described before [45 (link), 81 (link), 93 ]. Nuclei were stained with blue DAPI. Fluorescence intensity was measured using the Cytation 5 reader and ImageJ, and data were analyzed using Graph Pad Prizm.
Anti ck10
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-CK10 is a laboratory reagent used to detect and study the presence of the Cytokeratin 10 (CK10) protein in biological samples. CK10 is a member of the cytokeratin family of proteins that are found in epithelial cells. This antibody can be used in various laboratory techniques, such as Western blotting and immunohistochemistry, to identify and quantify CK10 expression in research applications.
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Lab products found in correlation
3 protocols using anti ck10
1
Secosteroids Modulate Keratinocyte Differentiation
2
Keratinocyte Differentiation and Antioxidant Response
3
Evaluation of Epidermal Keratinocyte Responses
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Human epidermal keratinocytes from the third passage were plated into 96-well plate and after reaching 60% confluency they were treated with 10 -7 M of either 1,25(OH) 2 D 3 , tachysterol 3 , 20(OH)T 3 or 25(OH)T 3 versus ethanol control for 24 h. Cells were fixed and stained with anti-catalase (Santa Cruz Biotechnology), anti involucrin (Santa Cruz Biotechnology) conjugated with Alexa Fluor 488, anti-CK10 (Santa Cruz Biotechnology), anti Mn-SOD (Millipore Corp) or anti-SOD1 (Santa Cruz Biotechnology), at dilutions of 1:100, 1:300, 1:100, 1:50 and 1:50, respectively, as described previously (22, 23, 48, 57) . For secondary antibodies, anti-goat (for CAT and SOD1), anti-mouse (for CK10) both with Alexa Fluor 488, or anti-rabbit with Alexa Fluor 660 (for MnSOD) were used, all at a dilution of 1:200. Images were taken at 10 x magnification using Cytation 5 reader. Nuclei were stained with blue DAPI. Fluorescence intensity was measured using the Cytation 5 reader and calculated using ImageJ software (National Institutes of Health).
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