Trc 501x

Clinical assessment included comprehensive ophthalmological examination and electrophysiological testing. Patients’ own and family medical history was registered regarding other ophthalmological disorders than eoHM as well as any systemic diseases. Best corrected visual acuity (BCVA) was recorded (Snellen chart) and refractive error expressed as spherical equivalent (SE). High myopia was specified as SE > − 6.0 dioptres (D) on at least one of the eyes. Slit lamp biomicroscopy with applantion tonometry and fundus ophthalmoscopy in mydriasis was carried out (Topcon SL-D701, Topcon, Tokyo, Japan). Digital fundus photography (TRC-501X; Topcon, Tokyo, Japan) and in some cases also ultra-wide field (200°) fundus images (Optos® California, Optos, Marlborough, MA) were taken. Spectral domain optical coherence tomography (macular scan) (Heidelberg Engineering, Heidelberg, Germany) was performed where possible. Axial length measurements were executed with an optical biometry system (IOLMaster 700, Carl Zeiss, Jena, Germany). Automated kinetic full-field perimetry was carried out with Humphrey Field Analyzer (Carl Zeiss Meditec, Jena, Germany).

Tail-retinal blood circulation time were performed respectively by FFA at the 1, 2 and 4 weeks after the operation. After fully dilating the pupils, each rat’s head was fixed in front of a contrast machine (TOPCON TRC-501X, Japan). The lens focal length and camera exposure times were adjusted to take color photographs of the fundus and no-red light images. The rats’ tail skin was sterilized and the 10% fluorescein sodium (Alcon, Fort Worth, TX, USA) was injected into the dorsal vein of the tail (40 mg/kg) at once. Blood flow was confirmed before the syringe needle was fixed with tape. The operator began timing and the assistant quickly pushed the sodium fluorescein injection. The recording frequency was set at 1 picture per second with a 30° field range and exposure level at 50, centered on the disc, to obtain the center or other quadrant images. The blood circulation time of the retinal arteries and veins in each rat was recorded.