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5 protocols using gold chloride haucl4 3h2o

1

Immunoassay Development for Mycotoxin Detection

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Alternariol (AOH), Alternariol monomethyl ether (AME), altenuene (ALT), tenuazonic acid (TeA), N, N-dicyclohexylcarbodiimide (DCC), N-hydroxysuccinimide (NHS), and gold chloride (HAuCl4·3H2O) were purchased from Sigma-Aldrich (Shanghai, China). Bovine serum albumin (BSA), human serum albumin (HSA), methyl 4-bromobutanoate, trisodium citrate dihydrate, and Tween-20 were purchased from Amresco (CochranSolon, OH, USA). PEG20000 was purchased from Alfa Aesar Chemical Co., Ltd. (Shanghai, China). Acetonitrile (HPLC grade) were purchased from Thermo Fisher Scientific (Waltham, NJ, USA). Incomplete Freund’s adjuvant, complete Freund’s adjuvant, 96-well microplates, goat anti-mouse IgG, and developing solution was offered by Beijing Kwinbon Biotechnology Co., Ltd. (Beijing, China). Polyvinyl chloride (PVC) sheet, NC membrane (Millipore 135), absorbent pad, conjugate pad, and sample pad were obtained from Shanghai Kinbio Tech. Co., Ltd. (Shanghai, China). Analytical grade sodium chloride (NaCl), dimethylformamide (DMF), methyl 4-bromobutanoate, potassium carbonate (K2CO3), ethanol, petroleum ether, tetrahydrofuran (THF), lithium hydroxide (LiOH), ethyl acetate (EtOAc), and other chemicals and solvents were ordered from Beijing Chemical Reagent Co. (Beijing, China). Water was deionized and purified with a Milli-Q system (Millipore, Bedford, MA, USA).
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2

Synthesis of Multivariate Nanoparticles

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All chemicals are commercially available with the highest purity and used without further treatment. Gold chloride (HAuCl4·3H2O) was purchased from Sigma-Aldrich; sodium hydroxide (NaOH), sodium borohydride (NaBH4), silver nitrate (AgNO3), zinc nitrate [Zn(NO3)2], copper nitrate [Cu(NO3)2], cadmium nitrate [Cd(NO3)2], erbium nitrate [Er(NO3)3], and GSH were purchased from Aladdin. Ultrapure water was used for all the experiments.
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3

Synthesis of Fluorescent Nanoparticles

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All chemicals and reagents were purchased from commercial sources and used without further purification. Gold chloride (HAuCl4·3H2O) was purchased from Sigma-Aldrich. Copper nitrate (Cu(NO3)2), Cadmium nitrate (Cd(NO3)2), 3-Mercaptopropionic acid (MPA), Sodium borohydride (NaBH4), Sodium hydroxide (NaOH), were purchased from Aladdin. Ultrapure water (18.2 MΩ*cm) was used for all the experiments. Kits and fluorescent probes were purchased from commercial sources and used as per the instructions.
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4

Synthesis of Metal Nanoparticles

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All chemicals are commercially available with the highest purity and used without further treatment. Gold chloride (HAuCl4·3H2O) was purchased from Sigma-Aldrich; sodium hydroxide (NaOH), sodium borohydride (NaBH4), copper nitrate (Cu(NO3)2), cadmium nitrate (Cd(NO3)2), and MPA were purchased from Aladdin. Ultrapure water (18.2 MΩ·cm) was used for all the experiments.
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5

Anticancer Effects of Letrozole and Berberine

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Letrozole powder (HPLC purity 99.12%) was kindly provided by Hetero drugs Pvt. Ltd., Hyderabad. DMSO (#PHR1309), camptothecin (#C9911), ethanol, berberine chloride, ascorbic acid, and gold chloride (HAuCl4.3H2O) were purchased from Sigma Aldrich (St. Louis, MO, USA) and used without additional purification. The MDA-MB-231 human breast adenocarcinoma cell line (NCCS, Pune) was used. The cell culture medium—high-glucose DMEM media (Cat. #: AL007)—fetal bovine serum (#RM10432), MTT reagent (5 mg/mL) (# 4060), and D-PBS (#TL1006) were purchased from Himedia, India. A 96-well plate for culturing the cells (from Corning, Bedford, MA, USA) and T25 flask (# 12556009, Biolite-Thermo, Waltham, MA, USA) were also used.
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