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Chloroform

Manufactured by GE Healthcare

Chloroform is a clear, colorless liquid commonly used in various laboratory applications. It is a versatile solvent with a high solubility for organic compounds. Chloroform's primary function is to serve as a medium for extraction and purification processes in analytical and research settings.

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2 protocols using chloroform

1

Synthesis and Antimicrobial Evaluation

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2-Ethylhexyl bromide was obtained from M/s. Sigma Aldrich Materials, 4-methoxyphenol (98%), acetic acid (99%) and potassium tert-butoxide (98%) were purchased from M/s. Avra, sodium hydride (Paraffin oil 60%) and hexane were provided by M/s. Lobal Chemie, ethyl acetate was received from M/s. Emplura, magnesium sulphate and para-formaldehyde were obtained from M/s. Fisher Scientific, hydrobromic acid (30–33% in acetic acid) was supplied by M/s. Spectrochem, Sodium bicarbonate was purchased from M/s. SDFCL, benzyl bromide (stabilized with propylene oxide) was provided by M/s. TCI, tetrahydrofuran was received from M/s. Rankem, methanol was supplied from M/s. SRL, whatman filter paper 40 ashless was obtained from M/s. GE Healthcare, N,N dimethylformamide and chloroform were purchased from M/s. Qualigens. Escherichia coli (ATCC 25922), Candida albicans (ATCC 10231), Staphylococcus aureus (ATCC 25923), and culture media were purchased from M/s. Himedia laboratories.
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2

Liposome Preparation and Labeling

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N-isopropylmethacrylamide (NIPMAM; Sigma-Aldrich), N,N′-methylenebisacrylamide (BIS; Fluka), hexadecyltrimethylammonium bromide (CTAB; Sigma-Aldrich), and 2,2′-Azobis(2-methylpropionamidine) dihydrochloride (V50; Fluka) were used as received. Styrene monomer (BASF) was purified on an Al2O3 column prior to use. The following were purchased from Avanti Polar Lipids: 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC, C44H84NO8P), 1,2-ditetradecanoyl-sn-glycero-3-phosphocholine (DMPC, C36H72NO8P), and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Liss Rhod PE, C68H109N4O14PS2). Alexa FluorTM 488 NHS Ester (C25H15Li2N3O13S2) was purchased from Thermo Fisher Scientific Inc. Cholesterol (C27H46O), chloroform (analytical grade, ≥ 99.8%), methanol (analytical grade, ≥ 99.9%), Mini Dialysis Kit (molecular weight cutoff, 8 kDa; GE Healthcare) were purchased from Sigma-Aldrich. MilliQ water was used in all the experiments.
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