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Fluorescein labeled anti mouse igg

Manufactured by Merck Group
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Fluorescein labeled anti-mouse IgG is a laboratory reagent used to detect and visualize mouse immunoglobulin G (IgG) in various experimental techniques. It is a conjugate of fluorescein, a fluorescent dye, and an antibody that specifically binds to mouse IgG. This product can be used to label and identify the presence of mouse IgG in samples, such as in immunoassays, immunocytochemistry, or flow cytometry.

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Close spelling variants of this product

Fluorescein-labeled goat anti-mouse immunoglobulin G (IgG) antibody Fluorescein Isothiocyanate (FITC)-labeled goat anti-mouse IgG Fluorescein isothiocyanate-labeled goat anti-mouse IgG Anti-IgG mouse IgG mouse

2 protocols using fluorescein labeled anti mouse igg

1

Pediatric Viral Respiratory Diagnostics

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Respiratory samples from hospitalized pediatric patients less than 1 year of age were submitted for routine viral diagnostics. Nasopharyngeal aspirates or swabs were obtained in a transport media (Hanks plus 2% FCS, penicillin, streptomycin, and amphotericin B). Samples were processed for antigen detection on pelleted cells by indirect immunofluorescence with monoclonal antibodies against RSV, adenovirus, influenza A and B and parainfluenza (EMD Millipore, Billerica, MA, USA) [12 (link),13 ]. A fluorescein labeled anti-mouse IgG was used (Sigma-Aldrich Corp., St. Louis, MO, USA). Readings were performed with a C. Zeiss microscope provided with epifluorescent equipment and a mercury lamp. An aliquot of the sample was preserved in liquid nitrogen.
Bose M.E., Shrivastava S., He J., Nelson M.I., Bera J., Fedorova N., Halpin R., Town C.D., Lorenzi H.A., Amedeo P., Gupta N., Noyola D.E., Videla C., Kok T., Buys A., Venter M., Vabret A., Cordey S, & Henrickson K.J. (2019). Sequencing and analysis of globally obtained human parainfluenza viruses 1 and 3 genomes. PLoS ONE, 14(7), e0220057.
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2

Rapid Viral Detection in Clinical Samples

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Nasopharyngeal aspirates or swabs were submitted for routine viral diagnostics in a transport media (Hanks plus 2% FCS, penicillin, streptomycin, and amphotericin B). Samples were processed for antigen detection on pelleted cells by indirect immunofluorescence with monoclonal antibodies against RSV, adenovirus, influenza A and B and parainfluenza (EMD Millipore, Billerica, MA, USA). A fluorescein labeled anti-mouse IgG was used (Sigma-Aldrich Corp., St. Louis, MO, USA). Readings were performed with a C. Zeiss microscope provided with epifluorescent equipment and a mercury lamp.
Bose M.E., He J., Shrivastava S., Nelson M.I., Bera J., Halpin R.A., Town C.D., Lorenzi H.A., Noyola D.E., Falcone V., Gerna G., De Beenhouwer H., Videla C., Kok T., Venter M., Williams J.V, & Henrickson K.J. (2015). Sequencing and Analysis of Globally Obtained Human Respiratory Syncytial Virus A and B Genomes. PLoS ONE, 10(3), e0120098.
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