Hsp70 antibody

4T1 cells were inoculated into 6‐well plates at a cell density of 1.6 × 10⁵ per well for overnight growth. The culture medium was exchanged with 1.6 mL of fresh medium containing 0–100 µg mL⁻¹pAu Pd HSs. After 6 h of incubation, the cells were irradiated with 808 nm laser (0.5 W cm⁻²) for 10 min and cultured for another 18 h. The control group without NIR laser irradiation was incubated at 37 °C for 24 h under dark conditions. The cells were lysed and collected with a cell lysis buffer and centrifuged to measure the protein content in the supernatant using the Bradford method. By calculation, the protein in each sample was quantified to 20 µg and 10% SDS‐PAGE gel was prepared for electrophoresis and transferred to a PVDF membrane. After sealing, β‐actin (1:1000, Beyotime), HSP70 antibody (1:1000, abcam), Caspase‐1 antibody (1:1000, Proteintech), or HO‐1 monoclonal antibody (1:1000, abcam) was co‐incubated with membranes for 12 h. After being washed with TBST solution, goat anti‐mouse horseradish peroxidase‐conjugated secondary antibody (1:1000, Beyotime) was added to incubate with the membrane for 1 h. After being washed 3 times with TBST buffer, the membrane was visualized by Bio‐Rad imaging system.