Anti murf1 antibody

Primary antibodies: anti-PSMD1 antibody (Abcam, Cambridge, UK, Ab140682, 1:15,000), anti-PSMB6 antibody (Abcam, Cambridge, UK, Ab3331, 1:1000), anti-PSMB3 antibody (Abcam, Cambridge, UK, Ab88665, 1:1000), anti-PSMA2 (Cell Signalling, MA, USA, #2455, 1:1000), Anti-Atrogin-1 antibody (Abcam, Cambridge, UK, Ab168372, 1:1000), and anti-MURF1 antibody (Abcam, Cambridge, UK, Ab96857, 1:20,000). Secondary antibodies: Rabbit-anti-mouse (DakoCytomation, Glostrup, Denmark P0260, 1:5000) and Goat-anti-rabbit (Jackson-ImmunoResearch Laboratories, West Grove, PA, USA 111-036-045, 1:10,000).

Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and penicillin-streptomycin were purchased from Welgene Inc. (Korea). Giemsa solution was purchased from Samchun Chemical Co., Ltd. (Korea), while 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), dexamethasone, and hematoxylin and eosin were purchased from Sigma-Aldrich (USA). In addition, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), TRIzol, bovine serum albumin (BSA), 3-hydroxy-3-methylbutyric acid (HMB), and xylene were obtained from Thermo Fisher Scientific (USA). SuPrimeScript RT Premix and AccuPower 2X GreenStar qPCR Master Mix were purchased from Genetbio Co., Ltd. (Korea) and Bioneer (Korea), respectively. Anti-myosin heavy chain (MyHC) antibody was purchased from R&D Systems (USA). Primary and secondary antibodies against MyoD, myogenin, atrogin-1, and FoxO3a were provided by Santa Cruz Biotechnology, Inc. (USA). Formaldehyde was purchased from Junsei Chemical Co., Ltd. (Japan). Paraffin was purchased from Leica Biosystems (Germany). The anti-MuRF1 antibody was purchased from Abcam (UK) and the anti-phosphorylated (p)-mTOR (Ser 2448), p-PI3K (Tyr 458), and p-Akt1/2/3 (Ser 473, Ser 474, Ser 472) antibodies were obtained from Cell Signaling Technology (USA). Finally, 3,3-diaminobenzidine (DAB) was purchased from Vector Laboratories Inc. (USA).