Glucose, Gly-Pro-p-nitroanilide, nicotinamide, dimethyl sulfoxide (DMSO), STZ, and sitagliptin phosphate were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Standard mouse/rat chow (Prolab® RMH2500, 5P14 diet) was purchased from PMI Nutrition International (MO, USA). Other chemicals were purchased from Sigma-Aldrich (St. Louis, MO). Proteins were quantified using the BCA protein assay kit (Pierce Biotechnology Inc., Rockford, IL, USA). The blood glucose content was determined using a glucose assay kit (Randox Laboratories, Ltd., Kearneysville, WV, USA). The blood insulin contents were determined by insulin ELISA kits (measurement range: 0.2–6.5 μg/L; 10–1247, Mercodia AB, Uppsala, Sweden).
Glucose assay kit
Manufactured by Randox
Sourced in United States
The Randox Glucose Assay Kit is a laboratory test used to quantitatively measure the concentration of glucose in a sample. The kit utilizes an enzymatic method to determine the glucose level.
Automatically generated - may contain errors
Lab products found in correlation
Sitagliptin phosphate, by Merck Group (1 mentions)
Nicotinamide, by Merck Group (1 mentions)
Gly pro p nitroanilide, by Merck Group (1 mentions)
Insulin elisa kit, by Mercodia (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Streptozotocin (stz), by Merck Group (1 mentions)
Bca protein assay kit, by Thermo Fisher Scientific (1 mentions)
Glycogen assay kit, by Abcam (1 mentions)
2 protocols using glucose assay kit
1
Glucose Tolerance Assay Protocol
2
Glycogen and Glucose Quantification from Fly Homogenates
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Three flies were homogenized in 200 µL PBS; the homogenates were then boiled for 10 min to inactive enzymes. The boiled samples were then centrifuged for 10 min at 4 °C at 15,000 × g to remove insoluble materials. One microliter of supernatant was used for glycogen quantification by using the Glycogen Assay kit (Abcam; ab65620). Twenty microliters of supernatant was used for glucose quantification by using Glucose Assay kit (Randox; GL2623). Three independent replicates (n = 9) per condition were used for both assays.
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