Qingkailing (QKL) was purchased from Shenwei Pharmaceutical Co., Ltd. (No. Z13020935, China). The protease inhibitor, radioimmunoprecipitation assay (RIPA) lysis buffer, and enhanced chemiluminescence (ECL) reagent were obtained from Vazyme Biotech (Nanjing, China). The antibodies against GAPDH, p-AMPK, and AMPK were purchased from Cell Signaling Technology (Danvers, MA USA), and the antibodies against NLRP3, ASC, IL-1β and caspase-1 were obtained from Abcam (Cambridge, UK).
Enhanced chemiluminescence ecl reagent
Manufactured by Vazyme
Sourced in China
Enhanced chemiluminescence (ECL) reagent is a laboratory product that generates a luminescent signal when combined with a target protein or biomolecule. It is used to detect and quantify the presence of specific proteins in a sample through Western blot analysis.
Automatically generated - may contain errors
Lab products found in correlation
P ampk, by Cell Signaling Technology (1 mentions)
Antibodies against nlrp3, by Abcam (1 mentions)
Antibodies against gapdh, by Cell Signaling Technology (1 mentions)
Il 1β, by Abcam (1 mentions)
Protease inhibitor, by Vazyme (1 mentions)
Hoechst 33342 staining kit, by Beyotime (1 mentions)
Antibody against β actin, by Proteintech (1 mentions)
Ripa lysis buffer, by Vazyme (1 mentions)
2 protocols using enhanced chemiluminescence ecl reagent
1
Qingkailing Modulates NLRP3 Inflammasome
2
Evaluating Mitochondrial Function in Cardiac Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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QSYQ were provided by Tasly Pharmaceutical Co., Ltd. (Tianjin, China) with the production batch number of 200402. The biochemical kits for detection of lactate dehydrogenase (LDH), malondialdehyde (MDA), glutathione (GSH) and tissue iron determination were obtained from Nanjing Jian Cheng Biotechnology Company (Jiangsu, China). The ELISA kits for detection of precursor brain natriuretic peptide (NT-pro BNP) and 4-hydroxynonenal (4-HNE) were obtained from Shanghai Heng Yuan Biotechnology Company (Shanghai, China). RIPA lysis buffer, protease inhibitor, and enhanced chemiluminescence (ECL) reagent were purchased from Vazyme Biotech Co., Ltd. (Jiangsu, China). Hoechst33342 staining kit was provided by Beyotime (Nanjing, China). Antibody against β-actin were provided by Proteintech Group (IL, USA) and antibody against PGC-1α, TFAM, Nrf1 were purchased from Santa Cruz (CA, USA). Antibody against FHC, GPX4, DRP1, phospho-DRP1, MFN-2 and OPA1 were obtained from Proteintech Group (IL, USA) and antibody against ACSL4 were obtained from Abclonal Proteinch (Wuhan, China).
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