An3ca

Manufactured by Procell

Sourced in China

The AN3CA is a laboratory equipment designed for general analytical purposes. It is a precision instrument that performs measurements and data analysis. The core function of the AN3CA is to provide accurate and reliable data for scientific research and testing applications.

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Lab products found in correlation

Rl95 2, by Procell (4 mentions) Ishikawa, by Procell (2 mentions) Hec 1 b, by Procell (2 mentions) Hec 1a, by American Type Culture Collection (1 mentions) Minimum essential medium (mem), by Meilun (1 mentions) U 2 os, by Meilun (1 mentions) Mccoy s 5a, by Meilun (1 mentions) Hek 293t, by Meilun (1 mentions) Dulbecco modified eagle medium (dmem), by Meilun (1 mentions) Talazoparib bmn 673, by Selleck Chemicals (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Hyclone, by Cytiva (1 mentions) Hec 1a, by Procell (1 mentions) Thp 1, by Procell (1 mentions) Fetal bovine serum (fbs), by Clark Bioscience (1 mentions) Ku 55933, by MedChemExpress (1 mentions) Olaparib, by MedChemExpress (1 mentions)

5 protocols using an3ca

Cell Culture Conditions for Cancer Studies

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The HEK-293T cell line (Cat No: TCH-C101) was obtained from Haixing Biosciences, Suzhou, Jiangsu, China. The endometrial carcinoma cell line (AN3 CA; Cat No: CL-0505) was obtained from Procell Life Science & Technology, Wuhan, Hubei, China. The endometrial carcinoma cell line (HEC-1-A) and human osteosarcoma cell line (U-2OS) were obtained from American Type Culture Collection (ATCC). HEK-293T was cultured in Dulbecco’s Modified Eagle Medium (D MEM, Meilunbio, China, Cat No: MA0212-2) with 10% Fetal Bovine Serum (FBS, Standard Quality, OriCell, China, Cat No: FBSST-01033). AN3 CA cell line was cultured in Minimum Essential Medium (MEM, Meilunbio, China, Cat No: MA0217) with 10% Fetal Bovine Serum (Standard Quality, OriCell, China, Cat No: FBSST-01033). U-2OS and HEC-1-A cell lines were cultured in McCoy’s 5A (Meilunbio, China, Cat No: MA0314) with 10% Fetal Bovine Serum (Standard Quality, OriCell, China, Cat No: FBSST-01033). All cells were grown at 37 °C with 5% CO₂.

Zhang H., Zhao Y., Wang J., Li J., Xia J., Lin Y., Zhong Y., Cao X., Jin J., Li X., Yang W., Ye M, & Jin X. (2023). FBXO7, a tumor suppressor in endometrial carcinoma, suppresses INF2-associated mitochondrial division. Cell Death & Disease, 14(6), 368.

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PTEN-proficient and PTEN-deficient EC cell lines

Cited 1 time

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KLE (PTEN-proficient) and Ishikawa, RL-95-2, or AN3CA (PTEN-deficient) EC cell lines were purchased from Procell Life Science & Technology Co. Ltd (Wuhan, China). Clinicopathological features, molecular profiles, short tandem repeat information, and culture conditions of EC cell lines are tabulated in SI 2A. Details of the sgPTEN-pSpCas9(BB)-2A-Puro (PX459) V2.0 construct are summarized in SI 2B. hBAD or hBADS99A (Ser99 mutated to Ala99) construct with a flag-tag is described in SI 2F. Based on the PARP-trapping capacities [42 (link)–45 (link)] (SI4A), three PARPis, Olaparib (specific to PARP1/2). Rucaparib (pan-PARP) or Talazoparib (specific to PARP1) were utilized. Olaparib (AZD2281), Rucaparib (AG-014699), and Talazoparib (BMN 673) were purchased from SelleckChem (Houston, TX, USA). All experiments were performed in 2% FBS with the respective medium.

Zhang X., Huang P., Wang L., Chen S., Basappa B., Zhu T., Lobie P.E, & Pandey V. (2022). Inhibition of BAD-Ser99 phosphorylation synergizes with PARP inhibition to ablate PTEN-deficient endometrial carcinoma. Cell Death & Disease, 13(6), 558.

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Culturing Endometrial Cancer and Immune Cells

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Human endometrial cancer cell lines (Ishikawa, HEC-1A, RL95-2, HEC-1B and AN3CA) and human mononuclear cells (THP-1) were purchased from Procell Life Science & Technology Co., Ltd. All cells were cultured in Dulbecco's modified Eagle's medium (Gibco; Thermo Fisher Scientific, Inc.) containing 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.), 100 µg/ml penicillin and 100 µg/ml streptomycin (HyClone; Cytiva) and cultured at 37˚C in 5% CO₂.

Gao J., Suo S., Li J., Wang C., Deng R., Hu Y, & Zhang C. (2023). IGFBP‑rP1 affects the proliferation, apoptosis and macrophage polarization of endometrial cancer cells by regulating the PI3K/AKT pathway. Experimental and Therapeutic Medicine, 25(4), 169.

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Culturing Human Endometrial Cancer Cell Lines

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The two human endometrial cancer cell lines RL95-2 and AN3CA and the reagents associated with cell culture were obtained from Procell (Wuhan, China). RL95-2 cells were cultured in DMEM/F12 medium and AN3CA cells were cultured in MEM-modified medium. All cells were supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 µg/mL streptomycin. In addition, the culture medium for RL95-2 cells was supplemented with 5 µg/mL insulin. The cells were incubated at 37 °C in 95% humidified air and 5% CO₂.

Tian S., Ren L., Liu C, & Wang Z. (2024). Atractylenolide II Suppresses Glycolysis and Induces Apoptosis by Blocking the PADI3-ERK Signaling Pathway in Endometrial Cancer Cells. Molecules, 29(5), 939.

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Endometrial Cancer Cell Lines Profiling

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This study used six human endometrial cancer cell lines, Ishikawa (type I), Hec-1B (type II), AN3CA (type I), KLE (type II) and RL952 (type I) which were purchased from Procell Life Science & Technology Co., Ltd., and Hec-108 (type I) was obtained from Shanghai Huiying biological technology Co., Ltd. Ishikawa and Hec-108 were cultured in DMEM (SH30243.01, Hyclone), while others were maintained in DMEM/F12 (SH30023.01, Hyclone). Both were supplemented with 10% FBS (FB15015, Clark) and 1% penicillin/streptomycin (100 U/mL). Cell cultures were incubated at 37 °C in a humidified incubator under 5% CO2.
Olaparib (HY-10162) and ATM inhibitor, KU55933 (HY-12016), were purchased from MedChemExpress, dissolved in 50 mM stocks in DMSO and stored at −20 °C. The antibodies used in this study, including ATM (92356), Phospho-ATM (5883), PARP (9532), Caspase-3 (9662), cleaved Caspase-3 (9664), b-actin (3700) and horseradish peroxidase (HRP)-linked anti-mouse (7076)/rabbit (7074) IgG, were all purchased from Cell Signaling Technology.

Zhang A., Zhang L., Xie X, & Liu D. (2023). Inhibition of ATM with KU-55933 Sensitizes Endometrial Cancer Cell Lines to Olaparib. OncoTargets and Therapy, 16, 1061-1071.

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