Cells were serum-starved overnight and treated with 100pM TGF-β for 60 minutes. Cells were lysed in 2× Laemmli sample buffer, and the proteins were resolved on a 10% SDS-PAGE gel. All primary antibodies (Slug #9585, p-Smad2 #3108, Smad2 #3103, p-Smad3 #9520, Smad3 #9523, p-ERK1/2 #9101, ERK1/2 #9102, p-AKT #4058, AKT #4691, p-p38 #4631, p38 #9212) were purchased from Cell Signaling (Danvers, MA, USA) except for β-actin (Sigma, AA5441) and N-cadherin (#610921) and E-cadherin (#610182) (BD Biosciences). Anti-mouse (#5470) and anti-rabbit (#5151) secondary antibodies were purchased from Cell Signaling. Blots were scanned using the LI-COR Odyssey (LI-COR Biosciences, Lincoln, NE, USA).
Anti mouse 5470 and anti rabbit 5151 secondary antibodies
Manufactured by Cell Signaling Technology
Anti-mouse (#5470) and anti-rabbit (#5151) secondary antibodies are laboratory reagents used to detect and visualize primary antibodies that were raised in mouse or rabbit, respectively. These secondary antibodies are conjugated with a reporter molecule, such as a fluorescent dye or an enzyme, to enable detection of the target protein in various experimental techniques like Western blotting, immunohistochemistry, and flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Erk1 2 9102, by Cell Signaling Technology (2 mentions)
Smad3 9523, by Cell Signaling Technology (2 mentions)
E cadherin, by BD (2 mentions)
P smad2 3108, by Cell Signaling Technology (2 mentions)
Smad2 3103, by Cell Signaling Technology (2 mentions)
N cadherin, by BD (2 mentions)
β actin, by Merck Group (2 mentions)
Akt 4691, by Cell Signaling Technology (2 mentions)
P38 9212, by Cell Signaling Technology (2 mentions)
P smad3 9520, by Cell Signaling Technology (2 mentions)
Slug 9585, by Cell Signaling Technology (2 mentions)
P akt 4058, by Cell Signaling Technology (2 mentions)
Perk1 2 9101, by Cell Signaling Technology (2 mentions)
2 protocols using anti mouse 5470 and anti rabbit 5151 secondary antibodies
1
TGF-β Signaling Pathway Activation
2
TGF-β Signaling Pathway Activation
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!