Hbp 1300 professional bp monitor

Weight was measured using a metric scale, calibrated to the nearest 0.1 kg, wearing light clothing without shoes. Height was measured using a tape measure, sensitive to the nearest 0.1 cm. Body mass index was then calculated as kg/m². Percent body fat was measured using the InBody720 (BioSpace, Seoul, Korea), an 8-point multifrequency bioelectrical impedance analyzer, while participants wore light clothing without shoes, socks, or metal. Researchers measured neck, waist, and hip circumference horizontally using a flexible, non-stretchable measuring tape, sensitive to the nearest 0.1 cm, by standard techniques per American College of Sports Medicine.
Waist (narrowest point of the torso), hip (largest circumference of the hips), and neck (just below the larynx and above the trapezius muscles) measurements were taken three times, as participants stood up straight with their feet together, head forward, and abdomen and shoulders relaxed. Resting blood pressure and heart rate were measured using the Omron HBP-1300 Professional BP Monitor (Omron, IL) after participants sat in a chair, legs uncrossed, and feet on the floor for 5 min. The cuff size was selected based on arm circumference and placed on the supported and relaxed upper right arm with the artery mark on the cuff in line with the brachial artery.

BP was measured twice using Omron HBP-1300 professional BP monitor. The second measure was recorded if the difference between two measures was less than 10 mm Hg. A third measure was performed if the difference of the first two measures was more than 10 mm Hg, and any two measures with a difference less than 10 mm Hg were recorded. Based on national data, BP reference standards for Chinese children and adolescents were established in 2010²⁵ , and the reference values of systolic blood pressure (SBP) and diastolic blood pressure (DBP) percentiles for Chinese children and adolescents were applied in this study, i.e., hypertension was defined as SBP and/or DBP ≥ 95^th percentile for age and gender.

Medical examinations are collected by trained medical personnel in accordance with standardized procedures. Weight and height were measured to the nearest 0.1 kg or cm, by a Seca 213 Portable Stadiometer Height-Rod and a Seca 877 electronic flat scale, respectively. Body mass index (BMI) was calculated as weight (kg) divided by height in square meters (m²). The waist circumference was measured with a tape measure with an accuracy of 0.1 cm. SBP (mmHg) and DBP (mmHg) were evaluated using an Omron HBP-1300 professional BP monitor. Venous blood was collected and divided into anticoagulant tube and serum separation tube. The blood samples in the serum separation tube were centrifuged at 3000× g immediately after blood collection for 15 min, 20–30 min after the blood was taken and then divided into serum aliquots and frozen at −80 °C for subsequent assays.
Serum fasting glucose, HDL-C, LDL, TC, TG, and UA were measured enzymically using an automatic biochemical analyzer (Hitachi 7600, Tokyo, Japan). Hb was determined by cyanide and high iron method, and HbA1c was determined by HPLC (Waters E2695, Milford, MA, USA). Element concentrations were measured by inductively coupled plasma mass spectrometry (ICP-MS, PerkinElmer, NexION 350, Waltham, MA, USA).