The black wheat seed was provided by the Cotton Research Institute of the Shanxi Academy of Agricultural Sciences (Taiyuan, China). The xylanase (food grade 50,000 U/g), cellulase (food grade 50,000 U/g), high-temperature α-amylase (food grade 40,000 U/g), and acid protease (food grade 700,000 U/g) were obtained from Solarbio (Beijing Solarbio Technology Co., Ltd., Beijing, China). The α-amylase (15 U/mg) and pepsin (474 U/mg) were purchased from Novozymes (Bagsvaerd, Denmark).
Pepsin
Manufactured by Novozymes
Sourced in Denmark
Pepsin is a proteolytic enzyme derived from porcine stomach mucosa. It is primarily used as a laboratory reagent for the digestion and hydrolysis of proteins. Pepsin functions by cleaving peptide bonds between amino acids, particularly those involving the carboxyl group of aromatic amino acids.
Automatically generated - may contain errors
Lab products found in correlation
Cellulase, by Solarbio (1 mentions)
Acid protease, by Solarbio (1 mentions)
Xylanase, by Solarbio (1 mentions)
α amylase, by Novozymes (1 mentions)
Bovine lactoferrin, by Merck Group (1 mentions)
Alkaline phosphatase alp assay kit, by Beyotime (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
Alizarin red solution, by Cyagen (1 mentions)
2 2 azinobis 3 ethylbenzthiazoline 6 sulfonate, by Merck Group (1 mentions)
3 protocols using pepsin
1
Enzymatic Pretreatment of Black Wheat
2
Mouse Osteoblast Cell Line Protocol
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The mouse osteoblast cell line MC3T3-E1 was obtained from the Institute of Biochemistry and Cell Biology (Chinese Academy of Sciences, Shanghai, China). Pepsin (EC 3.4.23.1; 2,500 U/mg of protein) was supplied by Novozymes (Beijing, China). The alkaline phosphatase (ALP) assay kit was purchased from Beyotime (Shanghai, China). Bovine lactoferrin was purchased from Sigma-Aldrich Co. (St. Louis, MO). The cell-counting kit 8 (CCK-8) was supplied by Dojindo (Japan). Alizarin red solution was obtained from Cyagen Biosciences Inc. (Suzhou, China) .
3
Chickpea Varieties' Antioxidant Potential
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Three varieties of chickpea, namely Kabuli type: Muying1 and Y2-514; Desi type: YZ-364, were collected from Xinjiang Agricultural Sciences. Bicinchoninic acid (BCA) protein assay kit was purchased from USA Pierce Company (Rockford, IL, USA). 1,1-Dipheny-2-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS) was bought from Sigma–Aldrich (St. Louis, MO, USA). The pepsin (3000 U/mg) and pancreatin from porcine pancreas (4000 U/mg) were bought from the Novozymes (Bagsværd, Denmark). All other chemicals and solvents used were of analytical grade.
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