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Ec epiplan neofluar 10x 0

Manufactured by Zeiss
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The EC Epiplan-Neofluar 10x 0.25 is a microscope objective lens manufactured by Zeiss. It provides a 10x magnification with a numerical aperture of 0.25. This lens is designed for reflected light microscopy applications.

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Lab products found in correlation

Lsm 710, by Zeiss (2 mentions) Ld c apochromat 1.1 w, by Zeiss (2 mentions) Illuminator hxp 200c, by Zeiss (1 mentions) Nis elements advanced research software, by Nikon (1 mentions) Axio examiner d1 microscope, by Zeiss (1 mentions) Andor solis software, by Oxford Instruments (1 mentions) Andor ixon ultra, by Oxford Instruments (1 mentions) Fl filter set 38 he gfp shift free, by Zeiss (1 mentions)

Spelling variants of this product

Epiplan-Neofluar (9 citations) EC Epiplan (8 citations) EC-Epiplan-NEOFLUAR (2 citations)

3 protocols using ec epiplan neofluar 10x 0

1

Live Imaging of Ferrofluid Labeling in Embryos

Cited 1 time
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Embryos were mounted for imaging in 0.8% low-melting agarose and imaged at 25 °C using a laser scanning confocal (LSM 710, Carl Zeiss Inc.). Images were taken every at 2.5 – 5 s (0.3 – 0.5 s) temporal resolution using a 40x water immersion objective (LD C-Apochromat 1.1W, Carl Zeiss Inc.) or a 10x air objective (EC Epiplan-Neofluar 10x 0.25, Carl Zeiss Inc.). Imaging of ferrofluid droplets in the embryo was done as previously reported20 (link). Ferrofluid droplets were fluorescently labelled using a custom-synthesized fluorinated Rhodamine dye33 (link), which was dissolved in the fluorocarbon-based ferrofluid oil at a final concentration of 37 μM.
Mongera A., Rowghanian P., Gustafson H.J., Shelton E., Kealhofer D.A., Carn E.K., Serwane F., Lucio A.A., Giammona J, & Campàs O. (2018). A fluid-to-solid jamming transition underlies vertebrate body axis elongation. Nature, 561(7723), 401-405.
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2

Live Imaging of Ferrofluid Labeling in Embryos

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Embryos were mounted for imaging in 0.8% low-melting agarose and imaged at 25 °C using a laser scanning confocal (LSM 710, Carl Zeiss Inc.). Images were taken every at 2.5 – 5 s (0.3 – 0.5 s) temporal resolution using a 40x water immersion objective (LD C-Apochromat 1.1W, Carl Zeiss Inc.) or a 10x air objective (EC Epiplan-Neofluar 10x 0.25, Carl Zeiss Inc.). Imaging of ferrofluid droplets in the embryo was done as previously reported20 (link). Ferrofluid droplets were fluorescently labelled using a custom-synthesized fluorinated Rhodamine dye33 (link), which was dissolved in the fluorocarbon-based ferrofluid oil at a final concentration of 37 μM.
Mongera A., Rowghanian P., Gustafson H.J., Shelton E., Kealhofer D.A., Carn E.K., Serwane F., Lucio A.A., Giammona J, & Campàs O. (2018). A fluid-to-solid jamming transition underlies vertebrate body axis elongation. Nature, 561(7723), 401-405.
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3

Fluorescent Antennae Imaging Protocol

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Antennae were imaged through a 10x (Zeiss EC Epiplan-Neofluar 10x/0.25) or a 50x (LD EC Epiplan-Neofluar 50x/0.55 DIC) objective mounted on a Zeiss Axio Examiner D1 microscope. For fluorescence, a light source (Zeiss Illuminator HXP 200C) and eGFP filter cube (FL Filter Set 38 HE GFP shift free) were used.
For image acquisition, an EMCCD camera (Andor iXon Ultra, Oxford Instruments), NIS Elements Advanced Research software (Nikon instruments), and Andor Solis software (Oxford Instruments) were used. Recordings were for 20 seconds, at a resolution of 512×512 pixels, and an exposure time of 200 ms (5 Hz).
Afify A., Betz J.F., Riabinina O., Lahondère C, & Potter C.J. (2019). Commonly used insect repellents hide human odors from Anopheles mosquitoes. Current biology : CB, 29(21), 3669-3680.e5.
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