The brains of the treated mice were collected and fixed with 4% paraformaldehyde for 72 h. Then, the SNpc tissues were embedded, and paraffin sections of 10 μm thickness were obtained. The block process was performed after antigen retrieval, in which the slides were incubated in 10% rabbit serum for 30 min at room temperature. Then, primary antibodies for TH (GB12181, Servicebio, China) and α-syn (ab212184, Abcam, China) were applied, and then the slices were washed and incubated with secondary antibodies. After counterstaining with DAPI for 10 min and sealing, the slides were observed using a panoramic section scanner.
Gb12181
Manufactured by Wuhan Servicebio Technology
Sourced in China
The GB12181 is a laboratory equipment product. It is designed for general laboratory use. No further details about its core function can be provided in an unbiased and factual manner without extrapolation.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using gb12181
1
Immunohistochemical Analysis of Midbrain Dopaminergic Neurons
2
Immunohistochemical Staining of TH
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Tissue paraffin slices were dewaxed in water and slices placed in box filled with 1 × citrate antigen repair buffer (pH 6.0) in a microwave oven for antigen retrieval. Next, the primary antibody (TH, Servicebio, GB12181, 1:1500) was added to the sections and incubated overnight.
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