A total of 5 μg of protein lysates were used to quantify Caspase3 (SEA626Mu, Cloud-Clone Corp.), TNFα (BMS607HS, eBioscience) and cAMP (KGE012B, R&D Systems) using manufacturer's instructions. The plates were measured on a FLUOstar Omega (BMG Labtech) at the recommended wavelength of the manufacturer. All assays were normalised against an ELISA against GAPDH (ab176642, Abcam). GAPDH was not changed in the proteomics and transcriptomics data and was therefore used for data normalisation. Six biological replicates were analysed in duplicates. The mean of each technical triplicate was normalised against the mean of the representative GAPDH technical replicates. Statistical analysis was performed via unpaired Student's t-test and the data are presented as fold-changes with the standard error of the mean (SEM).
Bms607hs
Manufactured by Thermo Fisher Scientific
The BMS607HS is a laboratory equipment product offered by Thermo Fisher Scientific. It is designed to perform specific functions in a laboratory setting. The core function of this product is to provide a controlled and consistent environment for various laboratory procedures. However, a detailed and unbiased description of the product's features and capabilities is not available at this time.
Automatically generated - may contain errors
Lab products found in correlation
Ab176642, by Abcam (2 mentions)
Sea626mu, by Cloud-Clone (2 mentions)
Kge012b, by R&D Systems (2 mentions)
Fluostar omega, by BMG Labtech (2 mentions)
Bms603hs, by Thermo Fisher Scientific (2 mentions)
Ab47328, by Abcam (1 mentions)
Anti rabbit igg, by Cell Signaling Technology (1 mentions)
Bms606 2, by Thermo Fisher Scientific (1 mentions)
Mlb00c, by R&D Systems (1 mentions)
Polytron pt10 35, by Kinematica (1 mentions)
5 protocols using bms607hs
1
Quantification of Apoptosis Markers
2
Serum Inflammation Marker Quantification
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Serum concentrations for the following inflammation markers were determined using ELISA following the manufacturer’s instructions: Serum Amyloid A (SAA, Tridelta, TP802-M), Lipopolysaccharide Binding Protein (LBP, Cell Sciences, CKM043), Interleukin-6 (IL-6, eBioscience, BMS603HS) and tumor necrosis factor-alpha (TNF-α, eBioscience, BMS607HS).
3
Quantifying Alzheimer's Biomarkers in Mouse Brain
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A total of 5 μg of protein lysates were used to quantify total CREB (KHO0231, Invitrogen), p-CREB (Ser133) (KHO0241, Invitrogen), Stathmin1 (E03S0217, Bluegene), Caspase3 (SEA626Mu, Cloud-Clone Corp.), cAMP (KGE012B, R&D Systems) and TNFα (BMS607HS, eBioscience) using enzyme-linked immunosorbent assay (ELISA) after manufacturer's instructions. P-Stathmin1 (Ser16) was quantified by using the Stathmin1 ELISA (E03S0217, Bluegene) and primary antibody against p-Stathmin1 (Ser16) (ab47328, Abcam) and secondary detector antibody (anti-rabbit IgG, HRP-linked) (#7074, Cell Signalling). The plates were measured on a FLUOstar Omega (BMG Labtech) at the recommended wavelength of the manufacturer. All assays were normalised against GAPDH (ab176642, Abcam) as it was not changed in the proteomics and transcriptomics data. Three biological replicates from hippocampus, neocortex, olfactory bulb and brainstem were analyzed in duplicates. The mean of each technical triplicate was normalised against the mean of the representative GAPDH technical replicates. Statistical analysis between APP/PS1 and age-matched wild-type mice was performed via unpaired Student's t-test and data are presented as fold-changes with the standard error of the mean (SEM).
4
Quantification of Inflammatory Cytokines
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5
Spinal Cord Injury Cytokine Analysis
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Concentrations of cytokines, including tumor necrosis factor alpha (TNF-α; BMS607HS; ThermoFisherScientific Inc., Waltham, MA), interleukin (IL)-1β (MLB00C; R&D Systems, Inc., Minneapolis, MN), and IL-10 (BMS614; ThermoFisherScientific), were measured in the spinal cord using the enzyme-linked immunosorbent assay (ELISA) by interpolation from a standard curve to investigate inflammatory/anti-inflammatory effects of ETZ in the acute phase after SCI. Spinal-cord samples were obtained from ∼10 mm spinal cord centered on the injured spinal cord in the saline and ETZ groups 7 days after surgery (Fig. 1C ). Immediately after euthanization, lesioned spinal cord segments were carefully removed and homogenized (POLYTRON PT 10-35; Kinematica AG, Malters, Switzerland) with RIPA Lysis and Extraction Buffer, protease inhibitors, and PhosStop inhibitors. An equal amount of protein was subjected to ELISA, which was performed according to the manufacturer's instructions. Absorbance was measured on a plate reader (Crocodile 5-in-one; Titertek-Berthold, Pforzheim, Germany) at 620 nm. Results are expressed as pg/mL of protein.
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