FISH was performed according to the protocol of FISH assay kit (Abnova, Taiwan). Briefly, cells were placed on the slides, which were sequentially treated with methanol:acetic acid, 2× standard saline citrate, hot citric acid, and pepsin. Centromere-specific α-satellite (CEN) probes for chromosomes 1 (green) and chromosome 5 (red) were added to each slide, the DNA and probe were co-denatured and hybridized, and the slides were washed and counterstained. Cells were then viewed with a Leica SP8 fluorescent microscope.
Fish assay kit
Manufactured by Abnova
The FISH assay kit is a laboratory equipment used for fluorescence in situ hybridization (FISH) analysis. The kit provides the necessary reagents and protocols to detect and localize specific DNA or RNA sequences within cells or tissue samples.
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Lab products found in correlation
2 protocols using fish assay kit
1
Fluorescence in-situ Hybridization Protocol
2
Chromosome Enumeration by FISH
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FISH was performed according to protocol of the FISH assay kit (Abnova, Taiwan). Briefly, cells were placed on slides, and sequentially treated with methanol, acetic acid, 2× standard saline citrate, hot citric acid, and pepsin. Centromere-specific α-satellite (CEN) probes for chromosomes 1 (Texas Red), and 5 (Cy5) were added to each slide, the DNA and probe were codenatured and hybridized, and the slides were washed and counterstained. Cells were then viewed with a Leica SP8 fluorescence microscope. For each chromosome, >30 nuclei were analyzed.
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