Il 12p70

Fluorescent-labeled anti-mouse mAbs APC-CD11c (N418), PE-CD40 (1C10), PE-CD80 (16-10A1), FITC-CD86 (GL1), FITC-MHCII (M5/114.15.2), PE-CCR7 (4B12), FITC-CD4 (GK1.5), APC-IL-4 (11B11), PE-IFN-γ (XMG1.2), or respective isotype controls were purchased from eBioscience (San Diego, CA, USA). Mouse CD36 blocking antibody [JC63.1] and control IgA antibody were from Abcam (Cambridge, USA). Rabbit anti-mouse pERK, ERK, pJNK, JNK, pP38, P38, IκBα, p65, LaminB1, GAPDH, goat anti-rabbit IgG-DY488 and goat anti-rabbit IgG-HRP were from Bioworld (St. Louis Park, MN, USA). RPMI 1640 medium, DAPI, FITC-OVA, and CFSE were from Invitrogen (Grand Island, NY, USA). Fetal bovine serum (FBS) was from Hyclone (Thermo, Melbourne, Australia). Recombinant GM-CSF, IL-4, IL-2, and CCL19 were from Peprotech (Rocky Hill, NJ). LPS (from Escherichia coli 026: B6), 2′,7′-dichlorodi hydro fluorescein diacetate (DCFDA) and β-carotene were from Sigma-Aldrich (St Louis, MO, USA). PD 98, 059, SP600125, BAY 11-7082, Bradford assay kit, Nitric Oxide assay kit, Superoxide dismutase assay kit, and Nuclear and Cytoplasmic Protein Extraction Kit were from Beyotime (Jiangsu, China). Mouse IL-6, IL-10, IL-12p70, TNFα ELISA Kit and Cell Counting Kit-8 (CCK8) were from Boster (Wuhan, China).

Dulbecco’s Modified Eagle Medium (12100046), RPMI-1640 (31800022), L-glutamine (A2916801), penicillin G (15140-122), streptomycin (15140-122), amphotericin B (15290-017), and all other cell-culture reagents were purchased from Gibco BRL Life Technologies (Grand Island, NY, USA). Concanavalin A (con A) (L7647) and dimethyl sulfoxide (DMSO) (D2650) were obtained from the Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). The Multiplex ELISA Kit For Mouse Cytokine (MEK1006, GMCSF, IFN-γ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-17, MCP-1, MIP-1α, RANTES, TNFα) was obtained from Boster Biological Technology (Pleasanton, CA, USA). Certified fetal bovine serum (FBS) (04-001-1A) was obtained from Biological Industries (Kibbutz Beit Haemek, Israel).