After 8 weeks of implantation, the rats were executed. The calvaria specimens, which included the implants, were collected and fixed in 4% paraformaldehyde. The samples were scanned using a high‐resolution Inveon micro‐computed tomography (micro‐CT; Siemens, Germany). Using the Inveon Research Workplace (Siemens, Germany) software, a three‐dimensional reconstruction and parametric analysis were conducted for the evaluation of bone formation within the bone defects. Following that, H&E and Masson staining were performed after decalcification.
Inveon micro computed tomography
Manufactured by Siemens
Sourced in Germany
The Inveon micro-computed tomography (micro-CT) is a lab equipment designed for high-resolution imaging of small samples. It uses X-ray technology to capture detailed three-dimensional images of the internal structure of various materials and specimens.
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Lab products found in correlation
3 protocols using inveon micro computed tomography
1
Bone Formation Evaluation via Micro-CT
2
Osteoporosis Model and Therapeutic Effects
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An OVX mouse
model was implemented according to previous studies.80 (link) Female eight week old C57BL/6J mice were randomly subjected
to bilateral oophorectomy and sham operation. Three months postoperation,
tail intravenous injection of apoVs was administered once a week,
using the equivalent vehicle (0.1 μm-filtered PBS) as a control.
Three months after supplementation, all mice were killed after being
anesthetized. Viscera and femurs were harvested. Viscera and left
femurs were fixed in 4% PFA for 1 week. To estimate the bone mass
and microarchitecture, micro-CT was executed by an Inveon microcomputed
tomography (Siemens, Germany) according to the following settings:
220 μA node current, 60 kV X-ray voltage, and 1500 ms exposure
time for each of 360 rotational steps. BMD and bone morphometric parameters
were determined by an Inveon Research Workplace (Siemens, Germany)
based on common guidelines.81 (link) Thereafter,
the samples were decalcified, embedded, sliced, and analyzed by histological
staining.
model was implemented according to previous studies.80 (link) Female eight week old C57BL/6J mice were randomly subjected
to bilateral oophorectomy and sham operation. Three months postoperation,
tail intravenous injection of apoVs was administered once a week,
using the equivalent vehicle (0.1 μm-filtered PBS) as a control.
Three months after supplementation, all mice were killed after being
anesthetized. Viscera and femurs were harvested. Viscera and left
femurs were fixed in 4% PFA for 1 week. To estimate the bone mass
and microarchitecture, micro-CT was executed by an Inveon microcomputed
tomography (Siemens, Germany) according to the following settings:
220 μA node current, 60 kV X-ray voltage, and 1500 ms exposure
time for each of 360 rotational steps. BMD and bone morphometric parameters
were determined by an Inveon Research Workplace (Siemens, Germany)
based on common guidelines.81 (link) Thereafter,
the samples were decalcified, embedded, sliced, and analyzed by histological
staining.
3
PLGA/pDA Scaffold-Mediated Bone Regeneration
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PLGA/pDA scaffold preparation was implemented as reported
previously.57 (link) PLGA/pDA scaffolds were immersed
in apoV solution at 4 °C for 12 h. To observe the distribution
of apoVs on scaffolds, apoVs were labeled with PKH26, and confocal
images were captured. To measure apoV release after immobilization,
we incubated the compound materials in saline at 37 °C and collected
supernatants at predetermined time intervals of 1–14 days.
BCA protein assay was applied to determine the protein content of
released apoVs. In situ, calvarial defects (5 mm
diameter) in Sprague–Dawley rats were constructed using a trephine
bur with copious saline irrigation. PLAG/pDA scaffolds loaded apoVs
were implanted in defects, with no scaffold and PLAG/pDA scaffolds
as control. Eight weeks postoperation, viscera and the whole calvarium
were harvested. Samples were fixed in 4% PFA for 1 week. To assess
bone formation, micro-CT was executed by an Inveon microcomputed tomography
(Siemens, Germany) according to the settings: 500 μA node current,
80 kV X-ray voltage, and 1500 ms exposure time for each of 360 rotational
steps. BMD and bone morphometric parameters were determined by an
Inveon Research Workplace (Siemens, Germany) based on common guidelines.81 (link) Thereafter, the samples were subjected to histological
examination.
previously.57 (link) PLGA/pDA scaffolds were immersed
in apoV solution at 4 °C for 12 h. To observe the distribution
of apoVs on scaffolds, apoVs were labeled with PKH26, and confocal
images were captured. To measure apoV release after immobilization,
we incubated the compound materials in saline at 37 °C and collected
supernatants at predetermined time intervals of 1–14 days.
BCA protein assay was applied to determine the protein content of
released apoVs. In situ, calvarial defects (5 mm
diameter) in Sprague–Dawley rats were constructed using a trephine
bur with copious saline irrigation. PLAG/pDA scaffolds loaded apoVs
were implanted in defects, with no scaffold and PLAG/pDA scaffolds
as control. Eight weeks postoperation, viscera and the whole calvarium
were harvested. Samples were fixed in 4% PFA for 1 week. To assess
bone formation, micro-CT was executed by an Inveon microcomputed tomography
(Siemens, Germany) according to the settings: 500 μA node current,
80 kV X-ray voltage, and 1500 ms exposure time for each of 360 rotational
steps. BMD and bone morphometric parameters were determined by an
Inveon Research Workplace (Siemens, Germany) based on common guidelines.81 (link) Thereafter, the samples were subjected to histological
examination.
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