6 phosphogluconic acid

Standard chemicals for 2D BN/SDS-PAGE and mass spectrometric analyses were purchased from Bio-Rad (Munich, Germany), USB Corporation (Cleveland, OH, USA), Merck (Darmstadt, Germany), and Sigma-Aldrich GmbH (Steinheim, Germany). B[a]P, dimethyl sulfoxide (DMSO), n-hexane (HPLC grade), and n-pentane (HPLC grade) were obtained from Merck. Fructose-6-phosphate and glucose-6-phosphate dehydrogenase were purchased from Alfa Aesar (Karlsruhe, Germany). 6-Phosphogluconic acid and glucose 6-phosphate were purchased from Sigma-Aldrich GmbH (Steinheim, Germany). NADP⁺ was obtained from Serva (Heidelberg, Germany). The NADP/NADPH Quantitation Kit was purchased from PromoKine GmbH (Heidelberg, Germany).

Glucose-6-phosphate (G6P), β-Nicotinamide adenine dinucleotide phosphate (NADP⁺), β-Nicotinamide adenine dinucleotide phosphate, reduced (NADPH), 6-phosphogluconic acid (6PG), adenosine-5′-triphosphate (ATP), adenosine diphosphate (ADP), β-Nicotinamide adenine dinucleotide (NAD), β-Nicotinamide adenine dinucleotide, reduced (NADH), DL-Dithiothreitol (DTT), nitroblue tetrazolium (NBT), phenazine methosulphate (PMS), blue dextran, bovine serum albumin (BSA), Sephacryl S-300, molecular weight marker kits for gel filtration, diethylaminoethyl-cellulose (DEAE-Cellulose), and chemicals for electrophoresis and isoelectric focusing (IEF) standard markers mixture pI 3.6–9.3 were purchased from Sigma Chemical Co. Molecular weight SDS marker proteins and 2′, 5′ ADP Sepharose 4B were products of Pharmacia Fine Chemicals Co.