Plasma VWF levels were quantified by enzyme-linked immunosorbent assay (ELISA) on day 10.
30 (link)
VWF levels in plasma of WT or ADAMTS13
−/−mice were calculated and shown as fold increase over pooled plasma VWF levels of untreated WT or untreated ADAMTS13
−/−mice, respectively. Interleukin (IL)-6 (Biolegend), IL-10 (R&D Systems), soluble P-selectin (sPsel; R&D Systems), and thrombin–antithrombin (TAT) complexes (Abcam) were measured by ELISA according to the manufacturer's instructions.