Anti bcl 2 antibody

MCF-7 cells, eagle’s minimum essential medium (EMEM), fetal bovine serum (FBS), Trypsin, and Dulbecco’s phosphate-buffered saline (DPBS) were obtained from ATCC (USA). Acetonitrile, acetone, isopropanol, ethanol, alpha-cyano-4-hydroxycinnamic acid (α-CHCA), human Bcl-2 protein, anti-Bcl-2 antibody, bovine serum albumin (BSA), and bovine insulin were purchased from Sigma-Aldrich (USA). Synthetic peptide FATVVEELFR (m/z 1210.6) and isotope labeled peptide FATVVEEL(13C15N)FR (m/z 1217.6) were purchased from GenScript USA Inc. Polydimethylsiloxane (PDMS) was purchased from Dow Corning Corporation (USA). Indium tin oxide (ITO) coated glass slides (100 ohm/sq) were obtained from NANOCS (USA). Tridecafluoro-1, 1, 2, 2-tetra-trichlorosilane (TTTS) was purchased from Gelest (USA). isopropanol and acetone were from VWR (USA). SU-8 2075 photoresist and SU-8 developer were from MicroChem (USA). Trypsin for protein digestion on the MIMAS platform was purchased from Promega (USA).

MCF-7 cells, eagle’s minimum essential medium (EMEM), and fetal bovine serum were obtained from ATCC (USA). Dulbecco’s phosphate-buffered saline (DPBS) buffer and the Hausser scientific hemocytometer were purchased from Thermo Fisher Scientific (USA). Acetonitrile, alpha-cyano-4-hydroxycinnamic acid (α-CHCA), human Bcl-2, anti-Bcl-2 antibody, bovine serum albumin (BSA), and bovine insulin were purchased from Sigma-Aldrich (USA). The peptide calibration mixture (angiotensin II 1046.5418 m/z [M+H]⁺, angiotensin I 1296.6848 m/z [M+H]⁺, substance P [1347.7354 m/z [M+H]⁺, bombesin 1619.8223 m/z [M+H]⁺, ACTH clip 1-17 2093.0862 m/z [M+H]⁺, ACTH clip 18-39 2465.1983 m/z [M+H]⁺, and somtostatin-28 3147.4710 m/z [M+H]⁺) was purchased from Bruker Daltonics Inc (USA). Isotope labeled peptides (FATVVEEL(d10)FR and FATVVEEL(13C15N)FR) were purchased from GenScript USA Inc. Polydimethylsiloxane (PDMS) was purchased from Dow Corning Corporation (USA). Indium tin oxide (ITO) coated glass slides (100 ohm/sq) were obtained from NANOCS (USA). Cr was obtained from SPI Supplies (USA). Tridecafluoro-1, 1, 2, 2-tetra-trichlorosilane (TTTS) was purchased from Gelest (USA). Isopropanol and acetone were from VWR (USA). SU-8 2075 photoresist and SU-8 developer were from MicroChem (USA). Ultrapure water was supplied from a synergy purification system (Millipore, USA).