Pe cf594 conjugated cd45 antibody

Manufactured by BD

The PE-CF594-conjugated CD45 antibody is a laboratory reagent used in flow cytometry applications. It binds to the CD45 surface antigen, which is expressed on most hematopoietic cells. The PE-CF594 fluorescent dye is covalently attached to the antibody, allowing for the detection and identification of CD45-positive cells.

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Lab products found in correlation

Draq5, by Cell Signaling Technology (2 mentions) Pe conjugated epcam antibody, by Cell Signaling Technology (2 mentions) Cellevent caspase 3 7 green detection reagent, by Thermo Fisher Scientific (2 mentions) Pe conjugated cd66b antibody, by STEMCELL (1 mentions) Alexa fluor 488 conjugated cd11b antibody, by STEMCELL (1 mentions) Clone g10f5, by STEMCELL (1 mentions) Calcein blue am, by Thermo Fisher Scientific (1 mentions) Clone hi30, by BD (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Hepes, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

CD45 PE-CF594 PE)-conjugated CD45 antibody CD45-PE

2 protocols using pe cf594 conjugated cd45 antibody

Multiparameter Imaging Flow Cytometry

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Cell viability, leukocyte activation, and platelet–leukocyte adhesion were studied using the ImageStream^X Mark II imaging flow cytometer (Amnis Corporation) equipped with a 40× objective, 6 imaging channels, and 405, 488, and 642 nm lasers. Whole blood was diluted 1:33 in RPMI 1640 medium (supplemented with 10 mM HEPES; Life Technologies) and stained with the following where applicable: calcein blue, AM (10 µM; Life Technologies); Pacific Blue-conjugated CD41 antibody (1:100; clone HIP8; BioLegend, Cat# 303713); CellEvent Caspase-3/7 Green Detection Reagent (5 µM; Life Technologies); Alexa Fluor 488-conjugated CD11b antibody (1:500; clone ICRF44; Stemcell Technologies, Cat# 60040AD); PE-conjugated CD66b antibody (1:150; clone G10F5; Stemcell Technologies, Cat# 60086PE); PE-conjugated EpCAM antibody (1:250; clone VU1D9; Cell Signaling Technology, Cat# 8995s); PE-CF594-conjugated CD45 antibody (1:666; clone HI30; BD Biosciences, Cat# 562279); and DRAQ5 (1 μM; Cell Signaling Technology). Single cells were gated using the nuclear stain DRAQ5. Viable cells were defined as calcein-positive but caspase-3/7-negative.

Wong K.H., Tessier S.N., Miyamoto D.T., Miller K.L., Bookstaver L.D., Carey T.R., Stannard C.J., Thapar V., Tai E.C., Vo K.D., Emmons E.S., Pleskow H.M., Sandlin R.D., Sequist L.V., Ting D.T., Haber D.A., Maheswaran S., Stott S.L, & Toner M. (2017). Whole blood stabilization for the microfluidic isolation and molecular characterization of circulating tumor cells. Nature Communications, 8, 1733.

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Imaging Flow Cytometry for CTC Analysis

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Imaging flow cytometry was performed using the ImageStream^X Mark II imaging flow cytometer (Amnis Corporation) equipped with a 40× objective, six imaging channels, and 405, 488, and 642 nm lasers. For analysis of cell viability and EpCAM expression, Brx cells obtained from culture or captured/released from our microfluidic device were resuspended in RPMI (supplemented with 0.3% BSA and HEPES) and stained with the following antibodies and stains where applicable: calcein blue AM (2.5 μM, ThermoFisher Scientific), CellEvent caspase 3/7 green detection reagent (1.75 μM, Life Technologies), PE-conjugated EpCAM antibody (1:260, clone VU1D9, Cell Signaling), PE-cf594-conjugated CD45 antibody (1:400, clone H130, BD Bioscience), and DRAQ5 (1 μM, Cell Signaling Technologies). Cells were acquired/gated using the nuclear marker DRAQ5. EpCAM positive cells were gated for viable cells (calcein positive and caspase 3/7 negative) versus dead cells (caspase 3/7 positive).

Park M.H., Reátegui E., Li W., Tessier S.N., Wong K.H., Jensen A.E., Thapar V., Ting D., Toner M., Stott S.L, & Hammond P.T. (2017). Enhanced Isolation and Release of Circulating Tumor Cells Using Nanoparticle Binding and Ligand Exchange in a Microfluidic Chip. Journal of the American Chemical Society, 139(7), 2741-2749.

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