Paraffin sections of mouse brain were deparaffinized, rehydrated, blocked in 1% H2O2 for 10 min and in 15% normal rabbit (for CD68) or goat (for GFAP) serums in PBS for 30 min; then incubated in a primary antibody overnight, on a shaker at 4°C (Rabbit anti-mouse GFAP antibody, Sigma-Aldrich, St. Louis, MO, 1:1000 dilution or rat anti-mouse CD68 antibody, Bio-Rad Laboratories, Hercules, CA, 1:2000 dilution). ImmPRESS Immunohistochemistry Kit (Vector Laboratories, Burlingame, CA) was used according to the manufacture’s protocol. After antigenic sites were visualized with DAB chromogen, sections were counterstained with hematoxylin.
Rabbit anti mouse gfap antibody
Manufactured by Merck Group
Sourced in United States
Rabbit anti-mouse GFAP antibody is a laboratory reagent used to detect the presence of the glial fibrillary acidic protein (GFAP) in mouse samples. GFAP is a cytoskeletal protein found in astrocytes and is commonly used as a marker for these cells. The rabbit anti-mouse GFAP antibody can be used in various immunodetection techniques, such as Western blotting and immunohistochemistry, to identify and quantify GFAP expression in mouse biological samples.
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2 protocols using rabbit anti mouse gfap antibody
1
Immunohistochemical Analysis of Mouse Brain
2
Stem Cell Isolation and Characterization
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The following reagents were purchased: Mesencult III medium (Stemcell Technologies, Vancouver, Canada); human lymphocyte separation buffer (Tianjin Haoyang Biotech Company, Tianjin, China); PE-labeled anti-human CD29 and CD34, and FITC-labeled anti-human CD44 and CD90 monoclonal antibodies (BD Biosciences, San Jose, CA, USA); recombinant human granulocyte colony-stimulating factor (Filgrastim; Amoytop Biotech Co., Ltd., Xiamen, Fujian Province, China); trypsin, bromide-oxyuridine reagent, rabbit anti-mouse bromide-oxyuridine antibody, lymphocyte separation medium, rabbit anti-mouse GFAP antibody, rabbit anti-mouse NSE antibody SABC kit, and the DAB chromogenic kit (Sigma, St. Louis, MO, USA); and brain-derived neurotrophic factor kit (Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd., Beijing, China).
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