Cells were transfected with 37.5 pM control siRNA or Peli1 siRNA (Santa Cruz Biotechnology) per manufacturer’s instructions. Transfected cells were grown in RPMI medium containing 10% FBS. qPCR was used to confirm the efficiency of Peli1 siRNA knockdown. At 24 h post transfection, cells were infected with ZIKV. Supernatants and cells were harvested at 96 h pi to measure viral load and cytokine production.
Peli1 sirna
Manufactured by Santa Cruz Biotechnology
Peli1 siRNA is a laboratory tool designed to temporarily suppress the expression of the Peli1 gene in cells. It is a synthetic double-stranded RNA molecule that can be introduced into cells to induce RNA interference, a process that inhibits the translation of the Peli1 mRNA and reduces the production of the corresponding protein. The core function of Peli1 siRNA is to provide researchers with a means to study the biological role of the Peli1 gene in various cellular processes.
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Lab products found in correlation
2 protocols using peli1 sirna
1
ZIKV Infection in Peli1-Silenced Cells
2
Peli1 Knockdown Impairs Angiogenesis
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Control small interfering RNA (C.siRNA; Cat# SC‐37007) and siRNA targeted against Peli1 (Peli1.siRNA; Cat# SC‐44401) were purchased from Santa Cruz Biotechnology, Inc (Dallas, TX). The HUVECs were treated with either C.siRNA or Peli1.siRNA (60 nmol/L) for 48 hours and then treated with VEGF. Matrigel with reduced serum (Cat# 354230, Corning Incorporated, Corning, NY) was dissolved at 4°C overnight, after which a μ‐Slide plate (ibidi USA, Inc, Madison, WI) was prepared with 10 μL Matrigel in each well and incubated at 37°C for 30 minutes. After incubation of the plates, 50 μL of treated cells (1.5×105 cells/mL) were seeded onto the gel. Tube formation was assessed after 8 hours of seeding using an Olympus QColor 3TM digital camera mounted on an Olympus BH2 microscope. Each well was photographed at ×200 magnification.11
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