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Cd138 pe 44f9

Manufactured by Miltenyi Biotec
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CD138 PE (44F9) is a fluorescently-labeled antibody that binds to the CD138 antigen. CD138, also known as syndecan-1, is a cell surface proteoglycan expressed on plasma cells and some epithelial cells. The PE (phycoerythrin) fluorophore is conjugated to the antibody, allowing detection and analysis of CD138-positive cells using flow cytometry or other fluorescence-based techniques.

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Lab products found in correlation

Sh800 sorter, by Sony (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (2 mentions) Cd3 percp cy5, by Thermo Fisher Scientific (2 mentions) Slamf7 apc 162, by BioLegend (2 mentions) Bcma pe cy7 19f2, by BioLegend (2 mentions) Cd14 apc cy7 mop9, by BD (2 mentions)

2 protocols using cd138 pe 44f9

1

Single-cell analysis of bone marrow cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bone marrow cells were stained with antibodies against either CD38 FITC (multi-epitope, Cytognos, 1:200), CD138 PE (44F9, Miltenyi, 1:100), SLAMF7 APC (162.1, Biolegend, 1:100), BCMA PE-Cy7 (19F2, Biolegend, 1:100), or with antibodies against CD45 FITC (HI30, eBioscience, 1:100), CD3 PerCP-Cy5.5 (OKT3, eBioscience, 1:100), CD14 APC-Cy7 (MoP9, BD Biosciences, 1:100), CD19 PE (HIB19, BioLegend, 1:100). Cells were stained with DAPI (1 μg/mL, Sigma-Aldrich) to exclude dead cells. Single cells were sorted into 96-well plates using a Sony SH800 sorter (Extended Data Fig. 1). Immediately after sorting, plates were spun down, and stored at –80 °C until further processing.
Frede J., Anand P., Sotudeh N., Pinto R.A., Nair M.S., Stuart H., Yee A.J., Vijaykumar T., Waldschmidt J.M., Potdar S., Kloeber J.A., Kokkalis A., Dimitrova V., Mann M., Laubach J.P., Richardson P.G., Anderson K.C., Raje N.S., Knoechel B, & Lohr J.G. (2021). Dynamic transcriptional reprogramming leads to immunotherapeutic vulnerabilities in myeloma. Nature cell biology, 23(11), 1199-1211.
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2

Single-cell analysis of bone marrow cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bone marrow cells were stained with antibodies against either CD38 FITC (multi-epitope, Cytognos, 1:200), CD138 PE (44F9, Miltenyi, 1:100), SLAMF7 APC (162.1, Biolegend, 1:100), BCMA PE-Cy7 (19F2, Biolegend, 1:100), or with antibodies against CD45 FITC (HI30, eBioscience, 1:100), CD3 PerCP-Cy5.5 (OKT3, eBioscience, 1:100), CD14 APC-Cy7 (MoP9, BD Biosciences, 1:100), CD19 PE (HIB19, BioLegend, 1:100). Cells were stained with DAPI (1 μg/mL, Sigma-Aldrich) to exclude dead cells. Single cells were sorted into 96-well plates using a Sony SH800 sorter (Extended Data Fig. 1). Immediately after sorting, plates were spun down, and stored at –80 °C until further processing.
Frede J., Anand P., Sotudeh N., Pinto R.A., Nair M.S., Stuart H., Yee A.J., Vijaykumar T., Waldschmidt J.M., Potdar S., Kloeber J.A., Kokkalis A., Dimitrova V., Mann M., Laubach J.P., Richardson P.G., Anderson K.C., Raje N.S., Knoechel B, & Lohr J.G. (2021). Dynamic transcriptional reprogramming leads to immunotherapeutic vulnerabilities in myeloma. Nature cell biology, 23(11), 1199-1211.
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