Anhydrous cobalt chloride (CoCl2) was obtained from Aladdin (Shanghai, China). Acriflavine (ACF) was purchased from MCE (Monmouth Junction, NJ, USA). Polyethylene glycol diacetate (COOH-PEG-COOH) was sourced from Melo PEG (Shanghai, China), and the CCK8 kit was acquired from ZOMANBIO (China, Beijing). PBS and Dulbecco’s modified Eagle’s medium (DMEM)were obtained from Gibco (Grand Island, NY, USA). Coomassie Blue Fast Staining Solution, PH fluorescent probe (BCECF AM), cell activity and cytotoxicity detection kit (CalceinAM/PI), reactive oxidative species (ROS) detection kit, and SDS-PAGE protein sample buffer (5×) were purchased from Beyotime (Shanghai, China). HIF-1α Rabbit mAb (RRID: AB_2799095), MMP-2 Rabbit mAb (RRID: AB_2799191), Bax Rabbit mAb (RRID: AB_10557411), and Caspase-3 Antibody (RRID: AB_331439) were obtained from Cell Signaling Technology (Danvers, MA, USA). N-Hydroxy succinimide (NHS) was sourced from ThermoFisher Scientific (Waltham, MA, USA). Tris-Glycine Running Buffer, Western Transfer Buffer, and 4% paraformaldehyde fixative solution were purchased from biosharp (Hefei, China). Lastly, the Cell-Light EdU Apollo567 In Vitro Kit was acquired from RIBOBIO (Guangzhou, China).
Cck8 kit
Manufactured by ZOMANBIO
Sourced in China
The CCK8 kit is a colorimetric assay used for the determination of cell viability and cytotoxicity. It measures the activity of living cells through the reduction of the tetrazolium salt WST-8 to a water-soluble formazan dye.
Automatically generated - may contain errors
Lab products found in correlation
Paraformaldehyde fixative solution, by Biosharp (1 mentions)
Bax rabbit mab, by Cell Signaling Technology (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Cell light edu apollo567 in vitro kit, by RiboBio (1 mentions)
Caspase 3 antibody, by Cell Signaling Technology (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
N hydroxysuccinimide, by Thermo Fisher Scientific (1 mentions)
2 protocols using cck8 kit
1
Cobalt Chloride-Acriflavine Cytotoxicity Evaluation
2
Viability Assay of SH-SY5Y Cells
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CCK-8 kit (ZOMANBIO Inc., ZP328, Beijing, China) was used to measure the viability of SH-SY5Y cells. A total of 1 × 10 4 cells in a volume of 100 µL per well were cultured in six replicate wells in a 96-well plate for 24 h and then treated with different concentrations of MnCl 2 (0, 62.5, 125, 250, 500, 1000 µM) for 3, 6, and 12 h. Then, 10 µL CCK-8 reagent was added per well with 90 µL DMEM and incubated for 1.5 h at 37 °C. The absorbance was determined at 450 nm using a microplate reader (Supplementary Fig. 1).
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