Copper (II) chloride dihydrate (CuCl2·2H2O), tannic acid (TA), sodium hydroxide (NaOH), and acetone were supplied from Aladdin Reagent Co., Ltd. (Shanghai, China). HSA was purchased from CSL Behring (Marburg, Germany). R848 and 3,3′,5,5′-tetramethylbenzidine (TMB) were provided by Macklin Biochemical Co., Ltd. (Shanghai, China). Soybean oil, 2′,7′-dichlorodihydro-fluorescein diacetate (H2DCFDA), [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl] tetrazolium bromide (MTT) and GOx from Aspergillus niger were purchased from Sigma–Aldrich Chemical Co., Ltd. (MO, USA). All chemical reagents were of analytical quality and were utilized without purification. Adenosine triphosphate (ATP) assay kit, 4,6-diamidino-2-phenylindole (DAPI), H2O2 assay kit, and glucose assay kit were obtained from Beyotime Biotechnology Co., Ltd. (Shanghai, China). Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) cell apoptosis kit was acquired from 4A Biotech. Co., Ltd. (Beijing, China). All flow cytometry antibodies were purchased from Biolegend (San Diego, CA, USA) or Bioss Co., Ltd. (Beijing, China) (Supporting Information Table S1 ). The enzyme-linked immunosorbent assay (ELISA) kits for cytokines were obtained from Biolegend (San Diego, CA, USA).
Enzyme linked immunosorbent assay elisa kits for cytokines
Manufactured by BioLegend
Sourced in United States
ELISA kits for cytokines are laboratory tools used to measure the concentration of specific cytokines in biological samples. These kits employ the enzyme-linked immunosorbent assay technique to quantify the amount of a target cytokine present in the sample.
Automatically generated - may contain errors
Lab products found in correlation
Soybean oil, by Merck Group (1 mentions)
Human serum albumin (hsa), by CSL Behring (1 mentions)
3 3 5 5 tetramethylbenzidine (tmb), by Maclin Biochemical Technology (1 mentions)
Gox from aspergillus niger, by Merck Group (1 mentions)
Glucose assay kit, by Beyotime (1 mentions)
2 7 dichlorodihydrofluorescein diacetate h2dcf da, by Merck Group (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Adenosine triphosphate atp assay kit, by Beyotime (1 mentions)
Phorbol 12 myristate 13 acetate pma ionomycin, by Merck Group (1 mentions)
Lipopolysaccharide lps, by Merck Group (1 mentions)
Amplex red hydrogen peroxide assay kit, by Thermo Fisher Scientific (1 mentions)
2 protocols using enzyme linked immunosorbent assay elisa kits for cytokines
1
Copper-Mediated Immunomodulatory Nanocomposite
2
Cell Culture Reagents and Assays
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Cell culture reagents including RPMI-1640 medium, fetal bovine serum (FBS), streptomycin and penicillin, 2-mercaptoethanol, and phosphate-buffered saline (PBS) were purchased from HyClone (Logan, UT, USA). Phorbol 12-myristate 13-acetate (PMA), ionomycin, and lipopolysaccharide (LPS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). MG-132 was purchased from MCE (Monmouth Junction, NJ, USA). Amplex Red Hydrogen Peroxide Assay Kit for measuring the level of ROS or MPO was purchased from Thermo Fisher (Carlsbad, CA, USA). Enzyme-linked immunosorbent assay (ELISA) kits for cytokines were purchased from BioLegend (San Diego, CA, USA).
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