For LXR activation assay, A549 cells were transfected with 0.75 µg of LXRE-driven luciferase reporter vector (LXRE-tk-Luc) and 0.75 µg of β-galactosidase control vector (Promega) using FuGENE HP transfection reagent. Six hours later, A549 cells were incubated with PLD for 12 h. The β-galactosidase enzyme activity was measured using the β-galactosidase Enzyme System (GloMax-96 Microplate Luminometer, Promega).
β galactosidase control vector
Manufactured by Promega
The β-galactosidase control vector is a plasmid that contains the lacZ gene, which encodes the β-galactosidase enzyme. This vector can be used as a positive control for experiments involving β-galactosidase expression or activity.
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Lab products found in correlation
2 protocols using β galactosidase control vector
1
LXR Activation Assay in A549 Cells
2
LXR Activation Luciferase Reporter Assay
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For LXR activation studies, 0.75 μg of LXRE-driven luciferase reporter vector (LXRE-tk-Luc) and 0.75 μg of β-galactosidase control vector (Promega) were used. The cells were transfected with vectors using FuGENE HP transfection reagent (Roche Applied Science, Indianapolis, IN, USA) according to the manufacturer's instructions. Six hours after transfection, cells were treated with SSa for 12 h. The β-galactosidase enzyme activity was determined using the β-galactosidase Enzyme System (Promega) according to the manufacturer' instructions. Luciferase activity was normalized by β-galactosidase activity.
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