The PLA in MBT2 cells and tissues of eyelids was performed using Duolink in situ PLA probe anti-mouse plus (Sigma-Aldrich Cat. No, Duo92001-30RXN), Duolink in situ PLA probe anti-rabbit minus (Sigma-Aldrich Cat, no. Duo92005-30RXN), Duolink in situ detection reagent green (Sigma-Aldrich Cat. no. 92014-30RXN) and Duolink in situ wash buffers, fluorescence (Sigma-Aldrich, Cat. no. Duo82049-4L) according to the manufacturer’s instructions. Antibodies used in this assay; mouse anti-GRHL3 (C-12) (Santa Cruz, Cat. no. sc-398838), rabbit anti-USP39 (Sigma-Aldrich, Cat. no. U0385), and rabbit anti-USP39 (Sigma-Aldrich, Cat. no. AV38825).
Rabbit anti usp39
Manufactured by Merck Group
Rabbit anti-USP39 is a laboratory reagent used in research applications. It is an antibody that specifically binds to the USP39 protein, which is a deubiquitinating enzyme involved in various cellular processes. The antibody can be used to detect and study the expression, localization, and function of USP39 in biological samples.
Automatically generated - may contain errors
1 protocol using rabbit anti usp39
1
Proximity Ligation Assay for GRHL3 and USP39
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