Immunostaining of BMDMs and mixed glial cells was performed as described previously (27 (link)). Immunofluorescent images of cells were visualized at room temperature using a confocal microscope (LSM 700, Zeiss) equipped with a 63×/NA 1.4 objective lens (Plan-Apochromat) and Zen acquisition and analysis software (Zeiss). The images were processed using Photoshop (Adobe) with minimal adjustment of brightness or contrast applied to the entire images.
Zen acquisition and analysis software
Manufactured by Zeiss
Zen acquisition and analysis software is a comprehensive imaging software platform developed by Zeiss. It provides tools for acquiring, processing, and analyzing microscope images and data. The software supports a wide range of Zeiss microscopes and imaging modalities, and offers a user-friendly interface for researchers and scientists.
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Lab products found in correlation
2 protocols using zen acquisition and analysis software
1
Immunostaining of BMDMs and Glia
2
Immunostaining of Neurons and Brain Tissue
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Immunostaining of cultured neurons and 100‐μm‐thick brain sections was performed as described 30 . Immunofluorescent images of cultured neurons were visualized at room temperature with a fluorescence microscope (Axioimage M2; Zeiss) equipped with a 20×/NA 0.80 (Plan‐Apochromat) objective lens and acquired using a cooled charge‐coupled device camera (Rolera EM‐C2; QImaging) with Zen software (Zeiss). For brain sections, neuronal images were captured at room temperature with a confocal microscope (LSM 700, Zeiss) equipped with a 20×/NA 0.80 (Plan‐Apochromat) objective lens and Zen acquisition and analysis software (Zeiss). The images were processed using Photoshop (Adobe) with minimal adjustment of brightness or contrast applied to the entire images. The camera lucida drawings were performed with ImageJ.
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