The full-length of human lncRNA CRNDE and POU2F1 was synthesized by Invitrogen (Shanghai, China) and cloned into the vector pmirGLO or pCDNA3 (Clontech Laboratories, Inc., San Francisco, CA). miR-539-5p mimics, inhibitor and negative control (NC) were synthesized by GenePharma (Shanghai, China). The cells were seeded in 6-well plates overnight (1 × 106/well), and then lncRNA (GenePharma), miRNA or plasmid were transfected with liposome Lipofectamine 2000 (Invitrogen) according to the instructions. The cells were changed to normal culture solution 6 h after transfection, 48 h and then proceed to the next test.
Pmirglo
Manufactured by Takara Bio
Sourced in China
The PmirGLO is a dual-luciferase reporter vector system designed for the analysis of microRNA (miRNA) activity. It contains a Firefly luciferase gene with a miRNA target sequence in the 3' UTR, and a Renilla luciferase gene for normalization. This system allows for the quantitative measurement of miRNA-mediated gene regulation.
Automatically generated - may contain errors
4 protocols using pmirglo
1
Overexpression of lncRNA CRNDE and POU2F1
2
Overexpression of lncRNA CRNDE and POU2F1
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The full-length of human lncRNA CRNDE and POU2F1 was synthesized by Invitrogen (Shanghai, China) and cloned into the vector pmirGLO or pCDNA3 (Clontech Laboratories, Inc., San Francisco, CA). miR-539-5p mimics, inhibitor and negative control (NC) were synthesized by GenePharma (Shanghai, China). The cells were seeded in 6-well plates overnight (1 × 10 6 /well), and then lncRNA (GenePharma), miRNA or plasmid were transfected with liposome Lipofectamine 2000 (Invitrogen) according to the instructions. The cells were changed to normal culture solution 6 h after transfection, 48 h and then proceed to the next test.
3
Overexpression of lncRNA CRNDE and POU2F1
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The full-length of human lncRNA CRNDE and POU2F1 was synthesized by Invitrogen (Shanghai, China) and cloned into the vector pmirGLO or pCDNA3 (Clontech Laboratories, Inc., San Francisco, CA). miR-539-5p mimics, inhibitor and negative control (NC) were synthesized by GenePharma (Shanghai, China). The cells were seeded in 6-well plates overnight (1×10 6 /well), and then lncRNA (GenePharma), miRNA or plasmid were transfected with liposome Lipofectamine 2000 (Invitrogen) according to the instructions.
The cells were changed to normal culture solution 6 h after transfection, 48 h and then proceed to the next test.
The cells were changed to normal culture solution 6 h after transfection, 48 h and then proceed to the next test.
4
Transfection of lncRNA and miRNA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The full-length of human lncRNA CRNDE and POU2F1 was synthesized by Invitrogen (Shanghai, China) and cloned into the vector pmirGLO or pCDNA3 (Clontech Laboratories, Inc., San Francisco, CA). miR-539-5p mimics, inhibitor and negative control (NC) were synthesized by GenePharma (Shanghai, China). The cells were seeded in 6-well plates overnight (1×10 6 /well), and then lncRNA (GenePharma), miRNA or plasmid were transfected with liposome Lipofectamine 2000 (Invitrogen) according to the instructions. The cells were changed to normal culture solution 6 h after transfection, 48 h and then proceed to the next test.
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