Tissue samples were fixed with formalin and embedded in paraffin. Sections were prepared for immunohistochemical staining and incubated with a primary antibody against ANXA2 (1:100; Abcam) for 1 h at 37°C and then incubated with a secondary antibody (Long Island, Shanghai, China) for 0.5 h at 37°C. The I scale of intensity was scored as : 0 (negative), 1 (weak), 2 (moderate), and 3 (strong) whereas the percentage of positive cells (P) in the area was used: 0, 1 (1-25%), 2 (26-50%), 3 (51-75%) and 4 (>75%). The multiplication of I and P resulted in the total score of 0-12 [17] .
Primary antibody against anxa2
Manufactured by Abcam
Sourced in China
Primary antibody against ANXA2. This antibody recognizes the ANXA2 protein, which is a calcium-dependent phospholipid-binding protein.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using primary antibody against anxa2
1
Immunohistochemical Quantification of ANXA2
2
Western Blot Analysis of ANXA2 in Panc-1 Cells
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For western blotting, cellular proteins were extracted from Panc-1 cells, separated by SDS-PAGE, and transferred onto a polyvinylidene fluoride membrane. A primary antibody against ANXA2 (1:500; Abcam) was used, and an anti-β-actin antibody (1:500, Abcam) was used as a control. The intensities of protein bands were analyzed quantitatively using Image-Pro Plus software.
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