Cell lysis, SDS-PAGE and Western blot analysis was performed as described previously (Arnold et al., 2015) . Following antibodies were used for detection of protein in lysates: anti-GAPDH (2118; Cell Signaling Technology, Danvers, MA, USA), anti-actin (A2066; Sigma-Aldrich), anti-myc (9B11, 2276; Cell Signaling Technology), anti-meprin β (polyclonal antibody, generated against a peptide of the ectodomain), anti-APP (N) (polyclonal antibody directed against N-terminus of APP; Thermo Fisher Scientific), anti-APP (C) (B63.2, polyclonal antibody directed against the C-terminus of APP), anti-CD9 (SC-13118; Santa Cruz, Dallas, TX, USA), anti-Flotillin 2 (SC-28320; Santa Cruz). Supernatants were used for analysis of sAPPα and Aβ (6E10, SIG-39320; Covance, Princeton, NJ, USA).
Anti app n
Manufactured by Thermo Fisher Scientific
Sourced in United States
Anti-APP (N) is a lab equipment product designed for the detection and analysis of the amyloid precursor protein (APP). It functions as a specific antibody that targets the N-terminal region of the APP molecule. This product is intended for research use only and its core function is to facilitate the study and understanding of APP-related processes and pathways.
Automatically generated - may contain errors
Lab products found in correlation
Anti meprin β, by Cell Signaling Technology (2 mentions)
Anti cd9 sc, by Santa Cruz Biotechnology (2 mentions)
Anti actin, by Merck Group (2 mentions)
Anti flotillin 2, by Santa Cruz Biotechnology (2 mentions)
Anti myc 9b11, by Cell Signaling Technology (2 mentions)
Anti gapdh, by Cell Signaling Technology (2 mentions)
2 protocols using anti app n
1
Protein Analysis via SDS-PAGE and Western Blot
2
Protein Analysis via SDS-PAGE and Western Blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell lysis, SDS-PAGE and Western blot analysis was performed as described previously (Arnold et al., 2015) . Following antibodies were used for detection of protein in lysates: anti-GAPDH (2118; Cell Signaling Technology, Danvers, MA, USA), anti-actin (A2066; Sigma-Aldrich), anti-myc (9B11, 2276; Cell Signaling Technology), anti-meprin β (polyclonal antibody, generated against a peptide of the ectodomain), anti-APP (N) (polyclonal antibody directed against N-terminus of APP; Thermo Fisher Scientific), anti-APP (C) (B63.2, polyclonal antibody directed against the C-terminus of APP), anti-CD9 (SC-13118; Santa Cruz, Dallas, TX, USA), anti-Flotillin 2 (SC-28320; Santa Cruz). Supernatants were used for analysis of sAPPα and Aβ (6E10, SIG-39320; Covance, Princeton, NJ, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!