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Fitc conjugated bovine lactadherin

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FITC-conjugated bovine lactadherin is a fluorescently labeled protein derived from bovine milk. It functions as a marker for the detection and visualization of phosphatidylserine, a component of cell membranes.

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Lab products found in correlation

Trucount tube, by BD (1 mentions) Thromboxane b2 eia kit, by Cayman Chemical (1 mentions) Chem8 cartridge, by Abbott (1 mentions) Kaolin, by Haemonetics (1 mentions) Anti histone h3 citrulline, by Proteintech (1 mentions) Anti mpo, by Proteintech (1 mentions)

Spelling variants of this product

Lactadherin (4 citations) Bovine lactadherin-FITC (3 citations) Bovine lactadherin (2 citations)

3 protocols using fitc conjugated bovine lactadherin

1

Platelet Phosphatidylserine and Aggregates Analysis

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To detect PLTs phosphatidylserine (PS) exposure, whole blood or PLTs isolated from healthy subjects and glioma patients were stained with fluorescein isothiocyanate (FITC)‐conjugated bovine lactadherin (Haematologic Technologies) and PerCP‐Cy5.5‐conjugated‐CD41 (Biolegend). To investigate PLTs‐neutrophil aggregates, isolated neutrophils from glioma patients and healthy donors were incubated with FITC‐conjugated‐CD41 (Biolegend) and APC‐conjugated‐CD66b (Biolegend) antibodies. The flow cytometry strategy is in Supporting Information Data S1.
Zhang S., Guo M., Liu Q., Liu J, & Cui Y. (2021). Neutrophil extracellular traps induce a hypercoagulable state in glioma. Immunity, Inflammation and Disease, 9(4), 1383-1393.
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2

Platelet Activation and Thrombosis Assays

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Calcium and kaolin used in thromboelastography were obtained from Haemonetics Corp (Braintree, MA). Multiplate agonists were obtained from Diapharma (West Chester, OH). FITC-conjugated bovine lactadherin was purchased from Haematologic Technologies, Inc (Essex Junction, VT). Gibco 1× Hanks Balanced Salt Solution (HBSS) without calcium and magnesium was obtained from Invitrogen Life Technologies (Carlsbad, CA). The following anti-human monoclonal mouse antibodies were purchased from BD Biosciences (San Jose, CA): APC-conjugated anti-CD62P (P-selectin), clone AK-4; PE conjugated anti-CD 154 (CD40 ligand or CD40L), clone TRAP1. PerCP conjugated mouse monoclonal anti-CD42b (glycoprotein Ib receptor, clone HIP-1 was purchased from BioLegend (San Diego, CA). For absolute particle counts TruCount tubes from BD Biosciences were utilized. Thromboxane B2 EIA kits were purchased from Cayman Chemicals (Ann Arbor, MI) and Human soluble CD40L (sCD40L) Extra Sensitive Platinum enzyme-linked immunosorbent assay (ELISA) kits were purchased from eBioscience (Vienna, Austria). CG4+ and CHEM8+ cartridges were purchased from Abbott Laboratories (Abbott Park, IL).
Reddoch K.M., Pidcoke H.F., Montgomery R.K., Fedyk C.G., Aden J.K., Ramasubramanian A.K, & Cap A.P. (2014). Hemostatic function of apheresis platelets stored at 4 °C and 22 °C. Shock (Augusta, Ga.), 41(0 1), 54-61.
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3

Platelet and Neutrophil Activation Assay

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To detect the phosphatidylserine (PS) exposure and CD62P(P‐selectin) of platelets, platelets isolated from healthy subjects and patients26 were stained with fluorescein isothiocyanate (FITC)‐conjugated bovine lactadherin (Haematologic Technologies, Essex Junction, VT) and anti‐CD62P antibody (Proteintech). Thereafter, isolated neutrophils from patients and healthy donors were incubated with FITC‐conjugated‐CD41 (Bioledgend) and the PE‐conjugated‐CD66b (Bioledgend) antibody before analysis using a flow cytometer, to investigate the presence of neutrophil‐PLTs aggregates. Moreover, isolated neutrophils were fixed and stained with anti‐Histone H3 citrulline (R2 + R8 + R17, ab5103) and anti‐MPO (Proteintech), followed by a goat antirabbit secondary antibody conjugated to Alexa Fluor 488 (Proteintech) and a goat antimouse secondary antibody conjugated to Alexa Fluor 647 (ab150115), before analysis using a flow cytometer, to detect the NETting neutrophils.36
Zhang S., Guo M., Liu Q., Liu J, & Cui Y. (2021). Neutrophil extracellular traps induce thrombogenicity in severe carotid stenosis. Immunity, Inflammation and Disease, 9(3), 1025-1036.
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