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Sabre xl

Manufactured by Stratec
Sourced in Germany

The Sabre XL is a high-performance analytical instrument designed for automated sample preparation and liquid handling. It features a modular design and advanced robotics to provide efficient and precise liquid handling capabilities for a wide range of laboratory applications.

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12 protocols using sabre xl

1

Femoral Bone Mineral Assessment

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The femora were thawed, cleaned of soft connective tissue, placed in a pDEXA scanner (Sabre XL; Norland Stratec, Pforzheim, Germany), and scanned using a pixel size of 0.1 × 0.1 mm2. The bone mineral content (BMC) and the area bone mineral density (aBMD) of the whole bone were determined with the software provided with the scanner. Quality assurance was performed by scans of the two solid-state phantoms provided with the scanner. The coefficient of variation (CV) of mice femoral aBMD is 2.4% in our laboratory.
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2

Bone Density Measurement by pDEXA

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Tibiae, femora, and the vertebral segments were scanned in a pDEXA scanner (Sabre XL, Norland Stratec, Pforzheim, Germany) at a pixel size of 0.1 mm × 0.1 mm and a scan speed of 3.0 mm/s as previously described in detail (Lodberg et al., 2015 (link)).
The coefficient of variation of femoral aBMD determined with pDEXA is 2.8% in our laboratory (same sample measured 10 times).
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3

Femoral Bone Density Measurement

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Whole femora were scanned in a DXA scanner (Sabre XL, Norland Stratec, Pforzheim, Germany) at a pixel size of 0.1 mm × 0.1 mm and a scan speed of 3.0 mm/s as previously described (23 (link)) to determine the areal bone mineral density (aBMD) and bone mineral content (BMC). Quality assurance was performed by scanning solid-state phantoms in accordance with the manufacturer’s instructions.
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4

Femur Bone Mineral Analysis

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The left femur was pDEXA scanned (Sabre XL, Norland Stratec, NY, USA) at a pixel size of 0.1 × 0.1 mm2. Bone mineral content (BMC) and area bone mineral density (aBMD) of the whole femur were determined (35 (link)).
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5

Femoral Bone Mineral Density Analysis

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After euthanization the right femora was exarticulated at the hip and the knee joint and stored in Ringer's solution at −20°C. The femora were thawed, dissected free of soft connective tissue, and placed in a standardized way in a peripheral DXA scanner (Sabre XL, Norland Stratec, Pforzheim, Germany) and scanned with a pixel size of 0.1 mm ×0.1 mm at a velocity of 2 mm/s. The areal bone mineral density (aBMD) was determined using the software provided with the scanner.
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6

Femoral Bone Mineral Analysis

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The length of the femora was measured with a digital sliding caliper, and the femora were then placed in a DEXA scanner (Sabre XL, Norland Stratec), and scanned with an isotropic pixel size of 0.1 mm. Bone mineral content (BMC) and areal bone mineral density (aBMD) were determined for the whole femur. Quality assurance was performed by scans of the two solid-state phantoms provided with the scanner.
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7

Femur Measurement and DEXA Scanning

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The length of the femur was measured with a digital sliding caliper, and thereafter placed in a DEXA scanner (Sabre XL, Norland Stratec) and scanned with a pixel size of 0.1 mm. The analysis included: Bone mineral content (BMC) and areal bone mineral density (aBMD) for the whole femur. Quality assurance was performed by scans of the two solid-state phantoms provided with the scanner.
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8

Femoral Bone Mineral Density Measurement

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The femora were DEXA scanned (Sabre XL, Norland Stratec, Pforzheim, Germany) using a pixel size of 0.5 mm × 0.5 mm. The areal Bone Mineral Density (aBMD) of the entire femora was determined using the scanner software. Quality assurance was performed by scans of the two solid-state phantoms provided with the scanner. The coefficient of variation (CV) of rat femoral aBMD is 2.8% in our laboratory (Thomsen et al., 2012 (link)).
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9

Femur Bone Density and Serum Biomarkers

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The left femur was pDXA scanned (Sabre XL, Norland Stratec) at a pixel size of 0.1 ​× ​0.1 ​mm2. Bone mineral content (BMC) and area bone mineral density (aBMD) of the whole femur were determined [31 (link)].
RANKL and osteoprotegerin (OPG) were measured in serum using commercially available ELISA kits (ab100749 and ab100733, Abcam), in accordance with the manufacturer’s instructions.
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10

Femoral DEXA Scanning in Adgra3 Mice

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Femora from 1-year-old Adgra3+/+ and Adgra3−/− females underwent densitometry using a DEXA scanner (Sabre XL, Norland Stratec, Pforzheim, Germany). The entire femur was scanned at a pixel size of 0.1 × 0.1 mm and a velocity of 3.0 m/s. The BMD was determined using the software supplied with the scanner. Quality control was conducted using two solid-state phantoms.
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