Cyt c

Manufactured by ABclonal

Sourced in China

Cyt-c is a laboratory reagent used for the detection and analysis of cytochrome c, a key protein involved in cellular respiration and apoptosis. It provides a reliable and sensitive tool for researchers to study these fundamental biological processes.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using cyt c

Protein Extraction and Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were lysed using the PRO-PREPTM Protein Extraction solution (iNtRon Biotech, Seongnam-Si, Republic of Korea). Total proteins were transferred to a polyvinylidene difluoride membrane and blocked with 5% skimmed milk for 1 h. Specific proteins were detected with primary antibodies: NLRP3 (A12694), GSDMD (A20197), IL-1β (A1112), caspase-3 (A19654), caspase-9 (A11451), BNIP3 (A5683), Cyt-c (A1561), Bax (A19684), Bcl-2 (A19693), β-actin (AC026), p-mTOR (S2448) (AP0115), mTOR (A2445), p-PI3K (AP0845), PI3K (A4992), p-Akt (AP0637), Akt (A17909), P62 (A19700), and LC3B (A19665), which were all bought from ABclonal Technology Co. Ltd., Wuhan, China. The blots were then treated with horseradish peroxidase-conjugated secondary antibodies. Bands were visualized using the ECL reagent and FluorChem M system (ProteinSimple, SFO, San Jose, CA, USA)

Dai X., Sun F., Deng K., Lin G., Yin W., Chen H., Yang D., Liu K., Zhang Y, & Huang L. (2022). Mallotucin D, a Clerodane Diterpenoid from Croton crassifolius, Suppresses HepG2 Cell Growth via Inducing Autophagic Cell Death and Pyroptosis. International Journal of Molecular Sciences, 23(22), 14217.

+ Open protocol

+ Expand

Synthesis and Application of LW-216 Compound

Check if the same lab product or an alternative is used in the 5 most similar protocols

LW-216 (C₃₀H₃₁NO₅, MW: 485.57, purity>96 %, CAS number: 2146090-18-6) was synthesized by Prof. Zhiyu Li in China Pharmaceutical University [22 (link)]. Auranofin (C₂₀H₃₄AuO₉, MW: 678.48, purity>99 %, CAS number: 34031-32-8) was obtained from Yuancheng Gongchuang Technology (Wuhan, China). Both LW-216 (10 mM) and Auranofin (1 mM) were dissolved in dimethyl sulfoxide (DMSO, Sigma-Aldrich, St.Louis, MO, USA) as a stock solution at -20 °C and diluted with medium before in vitro experiments. MG-132 (10 mM) and Cycloheximide (CHX, 10 mg/mL) were purchased from MedChemExpress (NJ, USA), which were both dissolved with DMSO. The final concentration of DMSO did not exceed 0.1 %. Primary antibodies for TrxR1, TrxR2, Ubiquitin, HA-Tag, Flag-Tag, Bax, Bcl-2, PARP1, Cleaved PARP1, Caspase-9, Cleaved Caspase-9, AIF, CytC, VDAC, Lamin A/C, Ki67, Cleaved Caspase-3, COL1A1 and β-actin were obtained from ABclonal Technology (Wuhan, China).

Wang R., Zhong L., Wang T., Sun T., Yang J., Liu X., Wu Y., Guo Q., Gao Y, & Zhao K. (2024). Inducing ubiquitination and degradation of TrxR1 protein by LW-216 promotes apoptosis in non-small cell lung cancer via triggering ROS production. Neoplasia (New York, N.Y.), 53, 101004.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!