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Mouse monoclonal anti pdcd6ip

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Mouse monoclonal anti-Pdcd6ip is a laboratory reagent used for the detection and analysis of the Pdcd6ip protein. It is a purified antibody derived from mouse cells that binds specifically to the Pdcd6ip protein.

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Lab products found in correlation

Seeblue plus2, by Thermo Fisher Scientific (2 mentions) Goat polyclonal anti tsg101, by Santa Cruz Biotechnology (2 mentions) Rabbit polyclonal anti asrgl1, by Proteintech (2 mentions) Rabbit monoclonal anti gls, by Abcam (2 mentions) Mouse monoclonal anti β actin, by Merck Group (2 mentions) Rabbit polyclonal anti calnexin, by Abcam (2 mentions) Rabbit polyclonal anti tom 20, by Santa Cruz Biotechnology (2 mentions) Mouse monoclonal anti gm130, by BD (2 mentions) Rat monoclonal and mouse monoclonal anti cd9, by BD (2 mentions)

2 protocols using mouse monoclonal anti pdcd6ip

1

Sucrose Gradient Fractionation of EVs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole NSCs, EVs and sucrose gradient fraction extracts were processed as described5 (link). Due to the limited amount of material obtained from sucrose gradient fractions, the correspondent WBs were performed using independent EV preparations starting from the same NSC batch. The following primary antibodies were used: mouse monoclonal anti-Pdcd6ip (BD transduction lab and Cell Signaling Technology); rat monoclonal and mouse monoclonal anti-CD9 (BD transduction lab and Life Technologies, respectively); goat polyclonal anti-TSG101 (Santa Cruz); rat monoclonal and mouse monoclonal anti-CD63 (MBL and Life Technologies, respectively); rabbit polyclonal anti-Asrgl1 (Proteintech); mouse monoclonal anti-GM-130 (BD transduction lab); rabbit polyclonal anti-calnexin (Abcam); rabbit polyclonal anti-Tom-20 (Santa Cruz); rabbit monoclonal anti-Gls (Abcam); mouse monoclonal anti-beta-Actin (Sigma). Molecular weight marker: SeeBlue Plus2 (Invitrogen). WB for mouse and human Cd63 and Cd9 were run under non reducing conditions, i.e sample buffer used for electrophoresis was devoid of reducing agent.
Iraci N., Gaude E., Leonardi T., Costa A.S., Cossetti C., Peruzzotti-Jametti L., Bernstock J.D., Saini H.K., Gelati M., Vescovi A.L., Bastos C., Faria N., Occhipinti L.G., Enright A.J., Frezza C, & Pluchino S. (2017). Extracellular vesicles are independent metabolic units with asparaginase activity. Nature chemical biology, 13(9), 951-955.
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2

Sucrose Gradient Fractionation of EVs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole NSCs, EVs and sucrose gradient fraction extracts were processed as described5 (link). Due to the limited amount of material obtained from sucrose gradient fractions, the correspondent WBs were performed using independent EV preparations starting from the same NSC batch. The following primary antibodies were used: mouse monoclonal anti-Pdcd6ip (BD transduction lab and Cell Signaling Technology); rat monoclonal and mouse monoclonal anti-CD9 (BD transduction lab and Life Technologies, respectively); goat polyclonal anti-TSG101 (Santa Cruz); rat monoclonal and mouse monoclonal anti-CD63 (MBL and Life Technologies, respectively); rabbit polyclonal anti-Asrgl1 (Proteintech); mouse monoclonal anti-GM-130 (BD transduction lab); rabbit polyclonal anti-calnexin (Abcam); rabbit polyclonal anti-Tom-20 (Santa Cruz); rabbit monoclonal anti-Gls (Abcam); mouse monoclonal anti-beta-Actin (Sigma). Molecular weight marker: SeeBlue Plus2 (Invitrogen). WB for mouse and human Cd63 and Cd9 were run under non reducing conditions, i.e sample buffer used for electrophoresis was devoid of reducing agent.
Iraci N., Gaude E., Leonardi T., Costa A.S., Cossetti C., Peruzzotti-Jametti L., Bernstock J.D., Saini H.K., Gelati M., Vescovi A.L., Bastos C., Faria N., Occhipinti L.G., Enright A.J., Frezza C, & Pluchino S. (2017). Extracellular vesicles are independent metabolic units with asparaginase activity. Nature chemical biology, 13(9), 951-955.
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