5 μg of recombinant mouse Tim-3 human IgG1 chimeric protein (rTim-3Fc) (R&D Systems, Minneapolis, MN) was added to 500 μL supernatant and immunoprecipitated with Protein A/G PLUS-Agarose (Santa Cruz Biotechnology, Dallas, TX). Proteins were resolved, western blotted, and incubated with rabbit anti-HMG1/2/3 (pAb) (Santa Cruz Biotechnology), biotinylated anti-human IgG (pAb) (SouthernBiotech, Birmingham, AL), HRP-linked anti-rabbit IgG (pAb) (Cell Signaling Technology, Danvers, MA), and streptavidin-HRP (R&D Systems) followed by visualization with SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, Rochester, NY).
Rabbit anti hmg1 2 3 pab
Manufactured by Santa Cruz Biotechnology
Rabbit anti-HMG1/2/3 (pAb) is a polyclonal antibody produced in rabbits that recognizes the high mobility group box (HMG) proteins HMG1, HMG2, and HMG3. This antibody is commonly used in research applications for the detection and study of these nuclear proteins.
Automatically generated - may contain errors
Lab products found in correlation
Streptavidin hrp, by R&D Systems (2 mentions)
Biotinylated anti human igg pab, by Southern Biotech (2 mentions)
Protein a g plus agarose, by Santa Cruz Biotechnology (2 mentions)
Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions)
Horseradish peroxidase hrp linked anti rabbit igg pab, by Cell Signaling Technology (1 mentions)
2 protocols using rabbit anti hmg1 2 3 pab
1
Immunoprecipitation of rTim-3Fc Protein
2
Immunoprecipitation and Immunoblotting of Tim-3
Check if the same lab product or an alternative is used in the 5 most similar protocols
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We added 5μg of recombinant (r) mouse Tim-3 human immunoglobulin G (IgG)1 chimeric protein (rTim-3Fc; R&D Systems, Minneapolis, MN) to 500 μL of supernatant and immunoprecipitated with Protein A/G PLUS-Agarose (Santa Cruz Biotechnology, Dallas, TX). Proteins were resolved, western blotted, and incubated with rabbit anti-HMG1/2/3 (pAb; Santa Cruz Biotechnology), biotinylated anti-human IgG (pAb; SouthernBiotech, Birmingham, AL), horseradish peroxidase (HRP)-linked anti-rabbit IgG (pAb; Cell Signaling Technology, Danvers, MA), and streptavidin-HRP (R&D Systems), followed by visualization with SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, Rochester, NY).
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