The tissue sections were processed and stained with primary antibodies for Mouse anti-Ets1 (Santa Cruz, Cat No. sc-55581; 1:200 dilution), Rabbit anti-DRP1 (CST, Cat No. 8570; 1:200 dilution) overnight at 4°C. Mouse tumor tissue sections of 5μm thickness were probed with Mouse anti-Ets1 (Santa Cruz, Cat No. sc-55581; 1:200 dilution), Rabbit anti-DRP1 (CST, Cat No. 8570; 1:200 dilution), Mouse anti-PCNA (CST, Cat No.2586, 1:200 dilution), Rabbit anti N-Cadherin (CST, Cat No. 13116, 1:200 dilution), Mouse anti e-Cadherin (CST, Cat No. 14472, 1:200 dilution) and Rabbit anti-Vimentin (CST, Cat No. 5741 , 1:200 dilution) overnight at 4°C. Anti-Rabbit Alexa fluor 568 (Invitrogen, Cat No. A21069) and Anti-Mouse Alexa fluor 488 (Invitrogen™, Cat No. A28175) based secondary antibody staining was performed at room temperature for 2 hours. Nuclear staining was done using DAPI for 5 mins. Images were acquired using Leica confocal microscope (TCS SP8, Buffalo Grove, IL, USA).
Mouse anti ets1
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The Mouse anti-Ets1 is a primary antibody that recognizes the Ets1 protein, a transcription factor involved in various cellular processes. This antibody can be used for the detection and analysis of Ets1 in a range of applications, such as Western blotting, immunoprecipitation, and immunohistochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Anti mouse alexa fluor 488, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 568 anti rabbit, by Thermo Fisher Scientific (1 mentions)
Rabbit anti drp1, by Santa Cruz Biotechnology (1 mentions)
Effectene transfection reagent, by Qiagen (1 mentions)
Protein a g beads, by Roche (1 mentions)
Carbobenzoxy leu leu leucinal, by Merck Group (1 mentions)
2 protocols using mouse anti ets1
1
Immunofluorescence Analysis of Tumor Markers
2
Regulation of Smad3 Ubiquitination in Hepatocytes
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Primary hepatocytes were treated with TGF-β1 for 6 h and then lysed in a buffer, as described in a previous study19 (link). For the ubiquitination assay, the hepatocytes were first transfected with shRNA adenovirus or plasmids using effectene transfection reagent (Qiagen, Germany) for 24 h, stimulated with TGF-β1 for 2 h, and finally treated with carbobenzoxy-Leu-Leu-leucinal (MG132, Sigma, USA) for 4 h. The lysates were incubated with antibodies against Smad3, p-Smad3 or p-Smad2 overnight, and then protein A/G beads (Roche, Switzerland) were added for 2 h. The precipitates were boiled for 5 min and were examined using immunoblot with mouse anti-Ets-1 (Santa Cruz, USA) or mouse anti-ubiquitin antibody (CST, MA, USA).
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